Evidence map›Paper›PMID 41619486›Full record

ArticlePoultry science2026

Synergistic effects of Moringa oleifera and Thymus vulgaris methanolic extracts on growth performance, Gut acid-base balance, Humoral immunity, and nutrient digestibility in broiler chickens.

Majid Ali, Hanan Alkhalaifah, Naila Chand, Shabana Naz, Rifat Ullah Khan, Ala Abudabos, Kasim Sakran Abass, Sohail Ahmad, Ibrahim A Alhidary

Abstract read
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Majid AliDepartment of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Hanan AlkhalaifahEnvironment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Safat, Kuwait. Electronic address: hkhalifa@kisr.edu.kw.
Naila ChandDepartment of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Shabana NazDepartment of Zoology, Government College University, Faisalabad, Pakistan. Electronic address: drshabananaz@gcuf.edu.pk.
Rifat Ullah KhanPhysiology Lab, College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan. Electronic address: rukhan@aup.edu.pk.
Ala AbudabosDepartment of Food and Animal Sciences, College of Agriculture, Tennessee State University, Nashville, TN 37209 USA. Electronic address: aabudabo@tnstate.edu.
Kasim Sakran AbassDepartment of Physiology, Biochemistry, and Pharmacology; College of Veterinary Medicine; University of Kirkuk, Kirkuk 36001, Iraq.
Sohail AhmadPhysiology Lab, College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan; Islamic University of Afghanistan, Kabul, Afghanistan.
Ibrahim A AlhidaryDepartment of Animal Production, College of Food and Agriculture Science, King Saud University, Riyadh, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated the effects of methanolic extracts of Moringa oleifera (MO) and Thymus vulgaris (TV), administered alone or in combination via drinking water, on growth performance, carcass traits, gut health, immune response, lymphoid organ development, liver and kidney function, and nutrient digestibility in broiler chickens. A total of 540 one-day-old Ross 308 broiler chicks were randomly allocated to six dietary treatments for a 42-day experimental period, including a basal diet control, a basal diet supplemented with a standard antibiotic growth promoter, MO extract alone, TV extract alone, and two combined MO-TV extract treatments. The combined treatments consisted of graded inclusion levels, with the most effective group receiving M50T50 (100mg/L MO + 150mg/L TV). The results demonstrated that combined MO and TV supplementation, particularly M50T50, significantly improved feed intake, body weight gain, and feed conversion ratio compared with the control and single-extract groups (p = 0.01). Dressing percentage and breast and thigh yields were highest in the combined extract groups (p = 0.01). Intestinal pH was significantly reduced, indicating an improved gut environment (p < 0.05), while antibody titers against infectious bronchitis (IB), Newcastle disease (ND), and infectious bursal disease (IBD) viruses were markedly elevated (p < 0.01). Lymphoid organ development, particularly bursal weight, was enhanced, whereas liver and kidney function markers remained within normal physiological ranges. Crude protein digestibility was also significantly increased (p = 0.01). These findings demonstrate that combined MO and TV methanolic extracts exert synergistic effects on growth performance, immunity, gut health, and protein utilization in broilers without adverse effects, highlighting their potential as a natural and effective phytogenic alternative to antibiotic growth promoters in poultry production.

Indexed as

ChickensDigestionImmunity, HumoralMoringa oleiferaPlant ExtractsThymus PlantAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsDietDietary SupplementsDrug SynergismMaleRandom AllocationPlant ExtractsBroilersGrowth performanceImmune responseMoringa oleiferaThymus vulgaris

Identifiers

PMID41619486
PMCPMC12874282

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.