Evidence map›Paper›PMID 41619307›Full record

ArticleChemistry & biodiversity2026

Biosynthesis of Silver Nanoparticles Using Black Pepper (Piper nigrum) Seed Extract and Evaluation of Their Cytotoxicity.

Nylton Ferreira Maciel, Gleice VasconcelosPereira do Lago, Glauce Vasconcelos da Silva Pereira, Ingryd Nayara de Farias Ramos, Aline Costa Bastos, Igor Alexandre Rocha Barreto, Nathiel Sarges Moraes, Marcos NazarenoLauné Dos Santos, João Augusto Pereira da Rocha, Alencar Kolinski Machado and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry & biodiversity, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Nylton Ferreira MacielPrograma De Pós-Graduação em Inovação Farmacêutica, Universidade Federal do Pará, Belém, Pará, Brazil.
Gleice VasconcelosPereira do LagoPrograma De Pós-Graduação em Ciência e Meio Ambiente, Universidade Federal do Pará, Belém, Pará, Brazil.
Glauce Vasconcelos da Silva PereiraPrograma De Pós-Graduação em Ciência e Meio Ambiente, Universidade Federal do Pará, Belém, Pará, Brazil.
Ingryd Nayara de Farias RamosNúcleo De Pesquisa em Oncologia, Universidade Federal do Pará, Belém, Pará, Brazil.
Aline Costa BastosNúcleo De Pesquisa em Oncologia, Universidade Federal do Pará, Belém, Pará, Brazil.
Igor Alexandre Rocha BarretoLaboratório De Mineralogia e Geoquímica Aplicada, Universidade Federal do Pará, Belém, Pará, Brazil.
Nathiel Sarges MoraesPrograma De Pós-Graduação em Ciência e Meio Ambiente, Universidade Federal do Pará, Belém, Pará, Brazil.
Marcos NazarenoLauné Dos SantosPrograma De Pós-Graduação em Ciência e Meio Ambiente, Universidade Federal do Pará, Belém, Pará, Brazil.
João Augusto Pereira da RochaInstituto De Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, Pará, Brazil.
Alencar Kolinski MachadoLaboratório De Cultura Celular e Efeitos Bioativos, Universidade Federal do Pará, Belém, Pará, Brazil.
Waldomiro Gomes Paschoal JuniorPrograma De Pós-Graduação em Física, Universidade Federal do Pará, Belém, Pará, Brazil.
Marcio Marcelo da Silva PessoaPrograma De Pós-Graduação em Inovação Farmacêutica, Universidade Federal do Pará, Belém, Pará, Brazil.
Maria de Nazaré Maciel UesugiPrograma De Pós-Graduação em Agronomia (PGAGRO), Universidade Federal Rural Da Amazônia, Belém, Pará, Brazil.
André Salim KhayatNúcleo De Pesquisa em Oncologia, Universidade Federal do Pará, Belém, Pará, Brazil.
Jorddy Neves CruzInstituto De Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.ORCID https://orcid.org/0000-0003-0529-3714
José de ArimatéiaRodrigues do RêgoPrograma De Pós-Graduação em Ciência e Meio Ambiente, Universidade Federal do Pará, Belém, Pará, Brazil.
Maria Regina Sarkis Peixoto JoelePrograma De Pós-Graduação em Ciência e Tecnologia De Alimentos, Universidade Federal do Pará, Belém, Pará, Brazil.
Davi do Socorro Barros BrasilPrograma De Pós-Graduação em Inovação Farmacêutica, Universidade Federal do Pará, Belém, Pará, Brazil.

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)
6 · The paper itself

Abstract

The green synthesis of silver nanoparticles (AgNPs) offers an environmentally friendly alternative to conventional methods by replacing toxic chemical reagents with natural sources. These methods utilize microorganisms, such as bacteria and fungi, or natural plant extracts rich in secondary metabolites that act as reducing and stabilizing agents during nanoparticle formation. In this study, we biosynthesized AgNPs using an ethanolic extract of Piper nigrum seeds and isolated piperine (PPN), the main bioactive compound. Furthermore, we evaluated the cytotoxic properties of PPN and its interaction with AgNPs (Np-AgPPN) in gastric cancer cells. The samples were characterized using UV-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR), scanning electron microscopy with energy-dispersive x-ray spectroscopy (SEM-EDS), x-ray diffraction (XRD), thermogravimetric (TG), and differential scanning calorimetry (DSC). Results suggest successful functionalization of AgNPs with piperine (Np-AgPPN). UV-Vis spectroscopy showed a hypsochromic shift of the maximum peak and a hypochromic effect, suggesting electronic interactions between PPN and Ag. FTIR-attenuated total reflectance (ATR) spectra revealed shifts in the carbonyl and aromatic bands, confirming PPN-Ag complex formation. SEM micrographs displayed rectangular crystals characteristic of PPN, whereas Np-AgPPN exhibited amorphous clusters. XRD analysis revealed a decrease in crystallinity and peaks characteristic of metallic Ag. Thermal analyses (TG/DTG and DSC) demonstrated the reduced thermal stability of Np-AgPPN compared to PPN, in addition to altered endothermic and exothermic transitions. In vitro cytotoxicity assays showed that Np-AgPPN had superior antitumor activity compared to PPN, with IC

Indexed as

Antineoplastic Agents, PhytogenicMetal NanoparticlesPiper nigrumPlant ExtractsSeedsSilverAlkaloidsBenzodioxolesCell Line, TumorCell ProliferationCell SurvivalDose-Response Relationship, DrugDrug Screening Assays, AntitumorGreen Chemistry TechnologyHumansParticle SizeAlkaloidsAntineoplastic Agents, PhytogenicBenzodioxolesPiperidinespiperinePlant ExtractsPolyunsaturated AlkamidesSilvercytotoxicityeco‐friendlynanoparticlespiperinesilver

Identifiers

PMID41619307
PMCPMC12860548

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.