Evidence map›Paper›PMID 41618557›Full record

ArticlePlant communications2026

The TaDof-2D-miR1832-TaP450-7A module regulates low-temperature-induced seed dormancy release in wheat.

Wei Gao, Zi-Heng Cui, Hua Xie, Jia-Jia Cao, Li-Tian Zhang, Yu-Xia Lv, Bing-Bing Tian, Chao-Xu He, Zi-Wei Wang, Pei-Bo He and 5 more

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Article in Plant communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Wei GaoKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Zi-Heng CuiKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Hua XieKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Jia-Jia CaoKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Li-Tian ZhangKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Yu-Xia LvKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Bing-Bing TianKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Chao-Xu HeKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Zi-Wei WangKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Pei-Bo HeKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Jie LuKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Chuan-Xi MaKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Cheng ChangKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China. Electronic address: changtgw@126.com.
Yong-Ling RuanInnovation Cluster of Crop Molecular Biology and Breeding, Anhui Agricultural University, Hefei 230036, China; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Horticulture, Northwest A&F University, Yangling 712100, China; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia. Electronic address: yong-ling.ruan@nwafu.edu.cn.
Hai-Ping ZhangKey Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, College of Agronomy, Anhui Agricultural University, Hefei 230036, China. Electronic address: zhhp20@163.com.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

In wheat, exposure to low temperatures (LTs) during the middle and late stages of seed development induces dormancy release; however, the underlying regulatory mechanism remains unclear. Here, using whole-transcriptome sequencing, we identified a novel microRNA (miR1832) that is downregulated by LT and located at a key node of the associated regulatory network. Germination assays showed that overexpression of miR1832 enhanced seed dormancy, whereas its silencing reduced seed dormancy. Sequence variation and association analyses further indicated that an A/G mutation at -670 bp in the miR1832 promoter is significantly associated with phenotypic variation in seed dormancy among wheat varieties, with the A allele correlated with strong dormancy. Through yeast one-hybrid, electrophoretic mobility shift assay, and dual-luciferase (LUC) reporter assays, we found that the LT-responsive Dof transcription factor TaDof-2D directly binds to the A site in the miR1832 promoter and inhibits its transcription. Subsequent expression analysis, dual-LUC assays, and 5' rapid amplification of cDNA ends confirmed that miR1832 targets the cytochrome P450 gene TaP450-7A, which is upregulated by LT and negatively regulates seed dormancy. Finally, physiological and biochemical analyses further demonstrated that the TaDof-2D-miR1832-TaP450-7A module participates in LT-induced dormancy release by modulating α-amylase activity and the abscisic acid and gibberellin pathways. These findings uncover a previously uncharacterized regulatory mechanism underlying LT-induced dormancy release and provide promising genetic resources and molecular markers for breeding wheat varieties with optimal dormancy levels.

Indexed as

Cold TemperatureCytochrome P-450 Enzyme SystemMicroRNAsPlant DormancyPlant ProteinsTriticumGene Expression Regulation, PlantGerminationSeedsCytochrome P-450 Enzyme SystemMicroRNAsPlant Proteinslow temperaturemicroRNApre-harvest sproutingseed dormancywheat

Identifiers

PMID41618557
PMCPMC13370231

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.