ArticleEuropean journal of medical research2026
Impact of mineral oil overlay on immature oocyte maturation in an optimized in vitro culture system.
Article in European journal of medical research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundTo assess the impact of oil overlay during maturation of immature oocytes in vitro in our optimized liquid culture system.
methodsWe conducted comparative analyses of pH, osmolality, and the levels of melatonin, FSH, E2, and hCG in the culture medium between oil-covered group (OC group) and non-oil-covered group (non-OC group). Furthermore, germinal vesicle (GV) oocytes were collected and matured into MII phase in our culture medium for 24 h. Subsequently, levels of ROS, SOD, MDA, and LDH were tested in OC group and non-OC group using either microplate assays or chemical fluorescence techniques. Finally, a total of 2432 immature oocytes obtained from controlled ovarian hyperstimulation (COH) cycles underwent in vitro maturation (IVM) in culture media with or without oil supplementation (1380 and 1052 oocytes, respectively), followed by insemination and embryo culture. The IVM prognostic indicators were assessed and compared between OC group and non-OC group.
resultsThere were no statistically significant differences in pH and osmolality values between the OC group and the non-OC group. However, the concentrations of melatonin, FSH, E2, and hCG in non-OC group were significantly elevated in the non-OC group compared to the OC group. Regarding oxidative stress parameters, levels of ROS, MDA, and LDH in the culture medium of the non-OC group were lower than those in the OC group, while SOD levels were higher in the non-OC group. Furthermore, the clinical outcome assessments revealed significantly higher rates of maturation, cleavage, and blastocyst formation in the non-OC group compared to the OC group, with no discernible difference in fertilization rates between the two groups.
conclusionOmission of mineral oil overlay enhances hormone bioavailability, diminishes oxidative stress, and significantly improves oocyte maturation and blastocyst formation rates in IVM culture.
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