Evidence map›Paper›PMID 41617720›Full record

ArticleNature communications2026

Engineered alcohol oxidases catalyse transesterification in aqueous media without competing hydrolysis.

Bin Wu, Yunjian Ma, Chenhao Feng, Limei Ren, Chiara Domestici, Yutong Wang, Thomas Hilberath, Ulf Hanefeld, Evgeny A Pidko, Frank Hollmann and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Bin WuSchool of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Yunjian MaSchool of Food Science and Engineering, South China University of Technology, Guangzhou, China. myj605740779@scut.edu.cn.ORCID 0000-0001-8003-8233
Chenhao FengSchool of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Limei RenDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Chiara DomesticiDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Yutong WangDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.ORCID 0000-0002-8790-4357
Thomas HilberathDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Ulf HanefeldDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands.ORCID 0000-0002-4102-6165
Evgeny A PidkoDepartment of Chemical Engineering, Delft University of Technology, Delft, The Netherlands.ORCID 0000-0001-9242-9901
Frank HollmannDepartment of Biotechnology, Delft University of Technology, Delft, The Netherlands. f.hollmann@tudelft.nl.ORCID 0000-0003-4821-756X
Yonghua WangSchool of Food Science and Engineering, South China University of Technology, Guangzhou, China. yonghw@scut.edu.cn.

Funding

EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) ERC, PeroxyZyme, No 101054658
6 · The paper itself

Abstract

Transesterification reactions are fundamental transformations in organic chemistry, yet performing them in aqueous media is challenging because of the competing hydrolysis reaction. In this study, we describe a mutant of alcohol oxidase from Phanerochaete chrysosporium (PcAOx-VPN) that also exhibits transesterification activity. Moreover, PcAOx-VPN displays no detectable hydrolytic activity, owing to its hydrophobic active site, which effectively excludes water. These characteristics make PcAOx-VPN a promising catalyst for transesterification reactions in aqueous media, a context that is typically compromised by competing hydrolysis.

Indexed as

Alcohol OxidoreductasesFungal ProteinsPhanerochaeteBiocatalysisCatalysisCatalytic DomainEsterificationHydrolysisProtein EngineeringWateralcohol oxidaseAlcohol OxidoreductasesFungal ProteinsWater

Identifiers

PMID41617720
PMCPMC12960964

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.