Evidence map›Paper›PMID 41615493›Full record

ArticleParasitology research2026

Spread and dynamics of Calicophoron daubneyi and Fasciola hepatica in an extensively kept water buffalo herd and suitability of an ELISA for detecting antibodies against F. hepatica.

Christoph Wenzel, Yury Zablotski, Gabriela Knubben-Schweizer, Christina Strube, Frank Weber

Abstract read
In one paragraph

Article in Parasitology research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Christoph WenzelClinic for Ruminants with Ambulatory and Herd Health Services, Ludwig-Maximilians-Universität München, 85764, Oberschleissheim, Germany. c.wenzel@lmu.de.ORCID http://orcid.org/0000-0001-6890-6245
Yury ZablotskiClinic for Ruminants with Ambulatory and Herd Health Services, Ludwig-Maximilians-Universität München, 85764, Oberschleissheim, Germany.ORCID http://orcid.org/0000-0001-6928-4089
Gabriela Knubben-SchweizerClinic for Ruminants with Ambulatory and Herd Health Services, Ludwig-Maximilians-Universität München, 85764, Oberschleissheim, Germany.ORCID http://orcid.org/0000-0002-0928-5933
Christina StrubeInstitute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559, Hannover, Germany.ORCID http://orcid.org/0000-0002-9093-1006
Frank WeberClinic for Ruminants with Ambulatory and Herd Health Services, Ludwig-Maximilians-Universität München, 85764, Oberschleissheim, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Water buffalo are ideal for grazing in restored wetlands due to their anatomical and behavioural adaptations. Unlike cattle or sheep, the water buffalo thrive in such habitats, feeding on rushes and reeds while helping maintain open landscapes. However, these moist environments also harbor risks, particularly parasitic infections by liver flukes (Fasciola hepatica) and the recently widespread rumen flukes, which require aquatic snails as intermediate hosts. For the present study faecal samples were taken once a year from 2016 to 2024 and monthly in two 12-month periods (2019/20 and 2022/23) from a water buffalo herd established in 2014 on a southern German farm and examined for fluke eggs with a sedimentation technique. In addition, climate data were obtained from a nearby weather station to analyse the impact of rainfall and sunshine hours on the fluke prevalence. To test whether blood samples can be used to detect infection with F. hepatica, residual quantities of blood samples from disease surveillance were tested for F. hepatica antibodies using a commercially available ELISA test. Copromicroscopical results showed that fluke prevalence rose significantly within a few years, with F. hepatica prevalence being always higher than C. daubneyi prevalence. Co-infections were common and seasonal patterns inconsistent. Fluke prevalence increased with rainfall and sunshine hours. In the herd, older water buffalo were more prone to liver flukes. The ELISA test for fascioliasis failed even though F. hepatica eggs were present in the faeces. The findings of the study highlight that extensively reared water buffalo are at high risk of trematode infection, which spreads rapidly throughout the herd and reaches high prevalence rates. The specific wallowing behaviour of water buffalo and favourable climatic conditions for parasite development may contribute to this development.

Indexed as

Antibodies, HelminthBuffaloesFasciola hepaticaFascioliasisTrematode InfectionsAnimalsEnzyme-Linked Immunosorbent AssayFecesGermanyPrevalenceAntibodies, HelminthBiodiversity managementHelminth infectionLiver flukeParamphistomesRumen flukeWetland grazing

Identifiers

PMID41615493
PMCPMC12868077

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.