Evidence map›Paper›PMID 41615325›Full record

ArticleACS infectious diseases2026

Structural Analysis of 23S rRNA Methylating Enzyme Cfr Reveals RNA-Binding Determinants for Methylation Regiospecificity and Antibiotic Resistance.

Michael Fruci, Annia Rodríguez Hernández, Tatiana Skarina, Lou Ann Verellen, Kaitlyn Tsai, Johanna M Virta, Danica Galonić Fujimori, Alexei Savchenko, Peter J Stogios

Abstract read
In one paragraph

Article in ACS infectious diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Natural Products AgainstMicroorganisms · 2026
    Review
  2. Structural Basis for CbioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Michael FruciLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada.
Annia Rodríguez HernándezDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94143, United States.
Tatiana SkarinaDepartment of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 1A4, Canada.
Lou Ann VerellenLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada.
Kaitlyn TsaiDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94143, United States.
Johanna M VirtaDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94143, United States.
Danica Galonić FujimoriDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94143, United States.ORCID 0000-0002-4066-9417
Alexei SavchenkoDepartment of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 1A4, Canada.ORCID 0000-0002-5256-9237
Peter J StogiosDepartment of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 1A4, Canada.ORCID 0000-0001-8663-1425

Funding

TO PROVIDE SCIENTIFIC SUPPORT TO THE CENTERS FOR RESEARCH ON STRUCTURAL BIOLOGY OF INFECTIOUS DISEASES.75N93022C00035 · NIAID · NORTHWESTERN UNIVERSITY AT CHICAGO · PI SATCHELL, KARLA · 2022 to 2025
$20.7M
Radical SAM-dependent methylation in antibiotic resistanceR01AI137270 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Danica Galonic Fujimori · 2018 to 2026
$3.8M
NIAID NIH HHS 75N93022C00035NIAID NIH HHS HHSN272201700060CNIAID NIH HHS R01 AI137270NIH HHS 75N93022C00035
6 · The paper itself

Abstract

The 23S rRNA methylating enzyme Cfr, found in pathogens including

Indexed as

Bacterial ProteinsDrug Resistance, BacterialEscherichia coli ProteinsMethyltransferasesRNA, Ribosomal, 23SAnti-Bacterial AgentsBinding SitesCrystallography, X-RayEscherichia coliKlebsiella pneumoniaeMethylationModels, MolecularProtein BindingRNA MethylationStaphylococcus aureusSubstrate SpecificityAnti-Bacterial AgentsBacterial ProteinsCFR protein, Staphylococcus aureusEscherichia coli ProteinsMethyltransferasesRNA, Ribosomal, 23SAlphaFoldantimicrobial resistancecrystallographymass spectrometrymethyltransferaseribosome

Identifiers

PMID41615325
PMCPMC12887959

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.