ArticleSmall (Weinheim an der Bergstrasse, Germany)2026
Thermo-Chemically Modified Silk Scaffolds Reveal Niche-Driven Regulation of Hematopoiesis and Fibrosis.
Article in Small (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
Abstract
Recreating the human bone marrow microenvironment in vitro remains a critical challenge in advancing our understanding of hematopoiesis and its disruption in disease. Here, we present a fully tunable bone marrow model based on silk fibroin scaffolds engineered through thermo-chemical processing to replicate the mechanical and structural features of native marrow. This 3D platform integrates mesenchymal stromal cells (MSCs) and supports the functional differentiation of hematopoietic stem and progenitor cells (HSPCs) into mature megakaryocytes and platelets. RNA sequencing of MSCs cultured on physiologically tuned scaffolds revealed transcriptional programs closely aligned with native stroma, validating the fidelity of the engineered niche. The model captures essential marrow dynamics, including matrix remodeling and perfusion flow, enabling direct assessment of thrombopoietic function. To simulate fibrotic remodeling, scaffolds were functionalized with TGF-β1, inducing MSC transition into myofibroblast-like cells and recreating pathological features of myeloproliferative neoplasms. In this context, patient-derived HSPCs exhibited impaired megakaryocyte maturation and aberrant calcium signaling, partially restored by interfering with calcium flux. To quantify microenvironment-driven dysfunction, we calculated changes in megakaryocyte size distribution using a Divergence Index. Combined with the engineered niche, this functional metric offers a powerful and quantifiable platform to dissect dysregulated hematopoiesis and evaluate therapeutic strategies in patient-derived systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.