ArticleJACS Au2026
Fc Profiling of Polyclonal IgG, IgA and IgM by Light Chain Capturing Coupled with NanoRP-LC-MS.
Article in JACS Au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proteoform profile of antibody Fc domains determines antibody effector functions, not only for biopharmaceuticals but also for endogenous antibodies. Endogenous immunoglobulin G (IgG) Fc-proteoforms have been well characterized by using different MS-based approaches, comprising bottom-up and intact Fc domain workflows. However, assessment of IgA1 and IgM Fc domains is still challenging, due to the more complex structure, and analyses have been limited to the peptide level only. In this work, a light-chain affinity capturing workflow combined with isotype-specific hinge-region digestion and subsequent intact Fc domain nanoRP-LC-MS analysis has been developed. The novel approach shows very good sensitivity and precision, enabling simultaneous capturing of antibody isotypes with sequential release and analysis of IgG, IgA1 and IgM Fcs from 10 μL of plasma. Single donor human samples were successfully analyzed, providing a comprehensive overview on Fc proteoforms but also on associated Fc-components such as the joining (J) chain of IgA and IgM and CD5L.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.