ReviewBiophysical reviews2025
Bioluminescent enzymatic biosensors: ways to manage their characteristics.
Review in Biophysical reviews, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- The Reductive Stress Nexus: Integrated Pathways of Metabolic Dysregulation, Senescence and Blood-Brain Barrier Failure in Neurodegenerative Disease and Brain Aging.Cell biochemistry and biophysics · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymatic biosensors have been lately gaining popularity in various areas of human life, from medicine to agriculture, owing to their effectiveness and selectivity towards the tested substances. Bioluminescent enzymatic biosensors attract special attention because of the light produced by the enzyme reaction. Researchers designing bioluminescent enzymatic sensors face a number of challenges, mainly associated with the low stability and/or sensitivity of the enzymes used as a biological recognition element of the biosensor. Herein we describe strategies of improving the analytical performance of bioluminescent enzymatic biosensors based on luciferase enzymes. We, first, discuss the increase in biosensors' sensitivity by optimizing the composition of the biological recognition element, including enzyme and substrate variations and addition of nanoparticles or stabilizing agents. Then, we provide insights into improving the stability of luciferases by immobilization or by the presence of chaperones and via directed mutagenesis. And the last section deals with projections for the future. We give special consideration to results of using these approaches in developing biosensors based on bioluminescent enzyme systems of fireflies and bacteria. Yet, the approaches described in the review are universal and can be effectively used to develop other enzymatic biosensors. Graphical Abstract:
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.