ArticleBiotechnologia2025
Gene expression, purification, and functional characterization of recombinant conotoxin μ-TIIIA and TIIIAlaMut in
Article in Biotechnologia, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Human and Marine Host Defense Peptides for Healthy Skin.Marine drugs · 2026Review
- Gene expression, purification, and functional characterization of recombinant conotoxin μ-TIIIA and TIIIAlaMut inBiotechnologia · 2025Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Conotoxins are small peptides known for their potent and selective activity on ion channels, offering potential applications in both medicine and cosmetology. This study aimed to design and validate recombinant conotoxin TIIIA and its mutant TIIIAlaMut, assess their biological activity on the voltage-gated Na Materials and methods: Fusion genes encoding Results: Both recombinant genes were successfully expressed, purified, and activated. Electrophysiological measurements demonstrated their ability to inhibit Nav1.4 channel activity, including the version extracted directly from the cream. In the human study, 47% of participants reported a visible reduction in wrinkles. Additional benefits included evening of skin tone, reduced erythema, and balanced sebum production in oily skin types. Conclusion: This study describes the design, bacterial expression, and functional analysis of recombinant conotoxins TIIIA and TIIIAlaMut. Their bioactivity was confirmed on human Nav1.4 channels. The recombinant toxins, including the form extracted from the cream, showed effects comparable to a synthetic standard. Application tests demonstrated the conotoxin's potential in cosmeceuticals, particularly in reducing periocular wrinkles and improving skin texture and tone.
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Registered trials
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