Evidence map›Paper›PMID 41613769›Full record

ArticleFrontiers in veterinary science2025

RPA-CRISPR/Cas12a-based detection of

Chaoqun Yan, Xiaozhen Li, Rulong Chen, Quanxin Wu, Youquan Zhuang, Na Li, Xuelian Ma, Yuefeng Chu, Huijun Shi, Qiang Fu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chaoqun Yan *College of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.
Xiaozhen Li *Urumqi Customs Technology Center, Urumqi, China.
Rulong ChenXinjiang Tianlai Agriculture and Animal Husbandry Group Co., Ltd., Bole, China.
Quanxin WuCollege of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.
Youquan ZhuangCollege of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.
Na LiCollege of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.
Xuelian MaCollege of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.
Yuefeng ChuCollege of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.
Huijun ShiCollege of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.
Qiang FuCollege of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.
Li YangCollege of Animal Medicine, Xinjiang Agricultural University, Urumqi, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: In this study, we developed a rapid, visual, and sensitive method for detecting Pm by combining recombinase polymerase amplification (RPA) with CRISPR/Cas12a. The PCR method based on kmt1 is the "gold standard" for studying Pm. So this assay targeted the kmt1 gene and was optimized for primer selection, reaction conditions, and crRNA/Cas12a ratios. Specificity verification was conducted through common respiratory pathogens, and sensitivity verification was carried out using plasmid dilution solutions. Results: The method showed a detection limit of 5 × 10

Indexed as

CRISPR/Cas12aPasteurella multocidaPCRRPAvisual detection

Identifiers

PMID41613769
PMCPMC12847050

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.