Evidence map›Paper›PMID 41613762›Full record

ArticleChemical & biomedical imaging2026

Silicon Rhodamine-Based Fluorescence Lifetime Probe for Dynamics Mapping Lysosomal Oxidative Stress.

Qingshuang Xu, Yiyan Zhang, Yuxun Tang, Senqiang Lv, Lianfeng Su, Pengfeng Mao, Pengqi Liu, Yutao Zhang, Chenxu Yan, Zhiqian Guo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical & biomedical imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Qingshuang XuZhonghang Monitoring & Testing Technology Research Institute, Hangzhou Iron & Steel Group Co., Ltd., Hangzhou 310022, China.
Yiyan ZhangShanghai Key Laboratory of Functional Materials Chemistry, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China.
Yuxun TangZhonghang Monitoring & Testing Technology Research Institute, Hangzhou Iron & Steel Group Co., Ltd., Hangzhou 310022, China.
Senqiang LvZhonghang Monitoring & Testing Technology Research Institute, Hangzhou Iron & Steel Group Co., Ltd., Hangzhou 310022, China.
Lianfeng SuZhonghang Monitoring & Testing Technology Research Institute, Hangzhou Iron & Steel Group Co., Ltd., Hangzhou 310022, China.
Pengfeng MaoZhonghang Monitoring & Testing Technology Research Institute, Hangzhou Iron & Steel Group Co., Ltd., Hangzhou 310022, China.
Pengqi LiuShanghai Key Laboratory of Functional Materials Chemistry, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China.
Yutao ZhangShanghai Key Laboratory of Functional Materials Chemistry, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China.
Chenxu YanShanghai Key Laboratory of Functional Materials Chemistry, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China.
Zhiqian GuoShanghai Key Laboratory of Functional Materials Chemistry, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China.ORCID https://orcid.org/0000-0002-2192-825X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lysosomes are organelles responsible for cellular degradation and recycling. The detection of changes in the lysosomal microenvironment, such as viscosity, oxidative stress, and pH value, as well as their interactions among dynamic organelles, remains an intriguing field that contributes to elucidating intracellular homeostasis. Here, we describe the development of a fluorescent probe tool for uniting fluorescence lifetime imaging microscopy (FLIM) and dual-channel near-infrared (NIR) fluorescence signals, which can simultaneously monitor viscosity and reactive oxygen species (ROS) in lysosomes. SiR-Eda exhibits a viscosity-dependent fluorescence lifetime and ROS-sensitive fluorescence emission, allowing for real-time tracking of lysosomal oxidative stress and viscosity within living cells. We demonstrate the utility of SiR-Eda in detecting changes in lysosomal viscosity and ROS in response to various stimuli including oxidative stress and lysosomal dysfunction. Our probe provides a convenient wash-free multifunctional tool for investigating lysosomal biology and has potential applications in the diagnosis and treatment of lysosome-related diseases.

Indexed as

dual-mode imagingfluorescence lifetimelysosomesprobeviscosity

Identifiers

PMID41613762
PMCPMC12848819

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.