Evidence map›Paper›PMID 41613437›Full record

ArticleFundamental research2025

Primary cilia in growth plates orchestrate long bone development.

Lei Zhang, Xiaoqiao Xu, Dike Tao, Xinyu Li, Pingping Niu, Xuyan Gong, Gongchen Li, Mengfei Yu, Yao Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fundamental research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lei ZhangShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Implantology, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China.
Xiaoqiao XuShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Implantology, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China.
Dike TaoShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Implantology, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China.
Xinyu LiShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Implantology, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China.
Pingping NiuShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Implantology, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China.
Xuyan GongShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Implantology, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China.
Gongchen LiShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Oral and Maxillofacial Surgery, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200092, China.
Mengfei YuStomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Zhejiang University, Hangzhou 311399, China.
Yao SunShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Implantology, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The growth plate plays a crucial role in long bone development and elongation, housing stem cells that contribute to bone formation. This study investigates the role of primary cilia, specialized organelles that regulate stem cell fate, in the development and repair of long bones. Here, we report the presence of primary cilia in all zones of the growth plate, particularly during embryonic development. Spatial transcriptomics and analysis of conditional knockout (CKO) mice identified that primary cilia mediate critical developmental signaling pathways within the growth plate. Disruption of primary cilia in growth plate chondroblasts or osteoblasts impaired long bone development by activating the Wnt signaling pathway and disrupting the cellular stemness. This resulted in elevated Mmp13 secretion, abnormal mineralization, and structural defects, ultimately hindering bone elongation. Time-lapse imaging showed an increased frequency of abnormal mitosis and a reduced rate of asymmetric division in skeletal stem cells (SSCs) from CKO mice. In conclusion, our findings indicated that primary cilia are critical for long bone development, regulating stem cell fate through key signaling pathways. Loss of primary cilia leads to excessive Wnt signaling and disruption of SSC stemness, impairing bone elongation. This study highlights the essential role of primary cilia in bone development and suggests potential therapeutic targets for skeletal disorders.

Indexed as

Growth plateLong bone developmentPrimary ciliaSkeletal stem cellWnt

Identifiers

PMID41613437
PMCPMC12848162

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.