ArticleJDS communications2025
Skimming and storage factors affect the detection of heat shock protein 70 in raw bovine milk.
Article in JDS communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Heat shock proteins (HSP) play a vital role in maintaining cellular function under elevated temperatures. Among these, heat shock protein 70 (HSP70) has recently been detected in bovine milk using competitive ELISA, highlighting its potential as a noninvasive marker of cellular stress and a tool for detecting heat stress (HS) in dairy cows. However, optimal management and storage conditions of milk samples for detection of HSP70 are yet to be determined. This study aimed to evaluate how different management and storage practices affect detection of HSP70 in milk samples. Samples were skimmed by centrifugation either before storage (SBS) or after storage (SAS), and then stored under different temperatures: (a) room temperature (T
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