ArticleJDS communications2025
Induction of intestinal barrier dysfunction in dairy heifers: Evaluation of new serum inflammatory markers and method for quantifying intestinal hyperpermeability.
Article in JDS communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Experimental induction of intestinal barrier dysfunction (e.g., inflammation and hyperpermeability) has been shown to induce a systemic inflammatory response and reduce productivity in lactating dairy cows. Because numerous natural situations on-farm (e.g., ruminal acidosis, heat stress, weaning) can impair intestinal barrier function, this is an important phenomenon to study. Therefore, our objective was to induce intestinal barrier dysfunction and evaluate new serum inflammatory markers as well as a new approach to measuring intestinal hyperpermeability. This was accomplished via oral aspirin administration for 21 d in 10-mo-old Holstein and Jersey heifers. Twelve heifers (6 each, Holsteins and Jerseys) were blocked by breed and then randomly assigned (3 heifers per breed) to receive either 0 or 200 mg aspirin/kg BW per day orally. At 0600 h on d 21 of the experiment, urine and blood samples were collected from each animal. Heifers then were dosed orally with gelatin capsules containing 50 g of Co-EDTA using a balling gun. Urine samples were subsequently collected at 1, 3, 6, 8, 12, 18, 24, 30, and 36 h after dosing. Urine samples were analyzed for Co and creatinine, and serum samples were analyzed for the inflammatory markers haptoglobin (Hp), LBP, FABP2, and TNF. Modeling urinary Co:creatinine ratios using a nonlinear function yielded an excellent fit and indicated that urinary Co excretion, a measure of intestinal permeability, was not increased due to aspirin but was higher for Jersey than for Holstein heifers. Also, serum concentrations of Hp and LBP were unaffected, but serum concentrations of FABP2 and TNF were increased due to aspirin administration. Finally, analysis of covariance indicated that serum TNF concentrations were highly correlated with urinary Co excretion. These data suggest that FABP2 and TNF may be valuable additional markers for the study of intestinal barrier dysfunction.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.