ArticleJDS communications2025
Comparison of glucose concentrations in samples frozen for varying durations and subjected to freeze-thaw cycles using 2 assay systems.
Article in JDS communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although laboratory best practices recommend that total storage time and number of freeze-thaw cycles should be minimized before analysis of metabolites within plasma, the specific impacts of storage time and freeze-thaw cycles on apparent glucose concentrations in bovine plasma have not been well quantified. Additionally, within the field of dairy science, multiple assay systems for glucose concentration analysis are used, yet these assays have not been directly compared with each other. Thus, the first objective was to investigate the stability of glucose in samples that have been frozen for an increased duration or frozen and thawed up to 4 times. The second objective was to investigate the agreement between 2 assay systems for glucose measurement: the peroxidase and glucose oxidase (PGO) assay system and the hexokinase reaction (HK) assay system. Blood samples were collected into sodium fluoride (NaF) tubes from 166 dairy cows, across different lactation stages, on 3 farms over a 2-d interval. After centrifugation, plasma was separated into 4 aliquots. One aliquot was used to determine the impacts of up to 4 freeze-thaw cycles, and the first measurement from this aliquot along with the other 3 aliquots were used to investigate potential glucose degradation in aliquots frozen for different lengths of time, including 2, 3, 4, and 16 wk. All samples were analyzed using both the PGO and HK assay systems. Bland-Altman plots, Pearson correlations, and paired
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