Evidence map›Paper›PMID 41611917›Full record

ArticleTranslational stroke research2026

Feasibility of a Multi-Laboratory Model of Middle Cerebral Artery Thromboembolic Stroke with Thrombolysis: TE-MCAo.

Michelle Lin, Mozammel Bhuiyan, Carly McCurry, Jessica Lamb, Marcio A Diniz, Karni Bedirian, Anil K Chauhan, Abhishek Jha, Aditi Jain, Enrique C Leira and 18 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational stroke research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Michelle LinDepartment of Neurosurgery, Keck School of Medicine of USC, Los Angeles, CA, USA.
Mozammel BhuiyanDepartment of Physiology and Neuroscience, Zilkha Neurogenetic Institute of the Keck School of Medicine of USC, Los Angeles, CA, USA.
Carly McCurryDepartment of Physiology and Neuroscience, Zilkha Neurogenetic Institute of the Keck School of Medicine of USC, Los Angeles, CA, USA.
Jessica LambDepartment of Physiology and Neuroscience, Zilkha Neurogenetic Institute of the Keck School of Medicine of USC, Los Angeles, CA, USA.
Marcio A DinizDepartment of Population Health Science and Policy, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Karni BedirianDepartment of Population Health Science and Policy, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Anil K ChauhanDepartment of Internal Medicine, Division of Hematology, Oncology and Blood & Marrow Transplantation, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Abhishek JhaDepartment of Internal Medicine, Division of Hematology, Oncology and Blood & Marrow Transplantation, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Aditi JainDepartment of Internal Medicine, Division of Hematology, Oncology and Blood & Marrow Transplantation, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Enrique C LeiraDepartments of Neurology & Neurosurgery, Department of Epidemiology, College of Public Health, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Rakeshkumar PatelDepartments of Neurology & Neurosurgery, Department of Epidemiology, College of Public Health, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Mohammad B KhanThe Medical College of Georgia at Augusta University, Augusta, GA, USA.
Pradip KamatThe Medical College of Georgia at Augusta University, Augusta, GA, USA.
David C HessThe Medical College of Georgia at Augusta University, Augusta, GA, USA.
Krishnan DhandapaniThe Medical College of Georgia at Augusta University, Augusta, GA, USA.
Huaxin ShengDepartment of Anesthesiology, Duke University, Durham, NC, USA.
Sasha ZhangDepartment of Anesthesiology, Duke University, Durham, NC, USA.
Wei YangDepartment of Anesthesiology, Duke University, Durham, NC, USA.
Bingren HuDepartment of Emergency Medicine and Neurosciences, University of California San Diego, San Diego, CA, USA.
Chunli LiuDepartment of Emergency Medicine and Neurosciences, University of California San Diego, San Diego, CA, USA.
Lauren SansingDepartment of Neurology, Yale University School of Medicine, New Haven, CT, USA.
Pinar CaglayanDepartment of Neurology, Yale University School of Medicine, New Haven, CT, USA.
Qinyue GuanDepartment of Neurology, Yale University School of Medicine, New Haven, CT, USA.
Ligia BoisserandDepartment of Neurology, Yale University School of Medicine, New Haven, CT, USA.
Cenk AyataMassachusetts General Hospital, Boston, MA, USA.
Takahiko ImaiMassachusetts General Hospital, Boston, MA, USA.
Kirsten LynchLaboratory of NeuroImaging, USC Mark and Mary Stevens Neuroimaging and Informatics, Institute of the Keck School of Medicine of USC, Los Angeles, CA, USA.
Patrick LydenDepartment of Neurosurgery, Keck School of Medicine of USC, Los Angeles, CA, USA. plyden@usc.edu.ORCID http://orcid.org/0000-0001-6170-4042

Funding

Testing Cerebroprotective Interventions with Rodent Ischemic Stroke ModelsU01NS130557 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HU, BINGREN · 2023 to 2025
$1.8M
The Role of Lysosomal Membrane Permeabilization and Cathepsin B Release in Stroke Brain InjuryR01NS129553 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Bingren Hu · 2023 to 2026
$1.5M
USC Neurosurgery Research and Education Training ProgramUE5NS099008 · NINDS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI William J Mack · 2024 to 2026
$126k
BLRD VA I01 BX005814NINDS NIH HHS R01 NS129553NINDS NIH HHS U01 NS130557NINDS NIH HHS UE5 NS099008
6 · The paper itself

Abstract

No single animal stroke model satisfies all needs of translational stroke investigation. While the nylon filament model is widely accepted in preclinical translational stroke research, thromboembolic models have more physiological relevance. Thromboembolic models are technically difficult, time consuming, and show variable results, making them problematic for multi-laboratory preclinical network use. We sought to develop and validate a multi-laboratory thromboembolic middle cerebral artery occlusion model that encompasses vessel occlusion and subsequent thrombolysis. To reduce the numbers of donor animals used, we developed a method to store donor blood for later use. Using prefabricated microcatheters, we simplified thrombus preparation and handling. Emboli were prepared in microcatheters and injected directly into the middle cerebral artery from the internal carotid artery. For thrombolysis we used intravenous Tenecteplase dosing at 1.5 mg/kg. To demonstrate feasibility and ease-of-use, the model was implemented at six research laboratories. We wrote and field-tested standard operating procedures, training videos, and hands-on surgical training workshops. We enrolled 170 Sprague Dawley rats of both sexes at six laboratories who performed 4 to 6 thrombus embolizations per week. All sites could achieve reproducible occlusion and thrombolysis. Thromboemboli prepared from stored blood served as well as emboli from freshly drawn blood. Of 135 rats who received one embolus, 33 (24%) died before 48-hour MRI scan. In survivors, stroke volume was 13 ± 16% of the ipsilateral hemisphere. Occlusions were seen in the MCA in 15%, distal ICA in 19%, both (T-occlusions) in 2% and in the MCA/ACA in 1%. Corner test, neurobehavioral battery, and MRI showed reasonably consistent stroke injury. We established the feasibility and reproducibility of a multi-laboratory rodent thromboembolic model but further development is needed to improve the success rate and lower the mortality rate before this model can be accepted widely.

Indexed as

Disease Models, AnimalFibrinolytic AgentsInfarction, Middle Cerebral ArteryThrombolytic TherapyAnimalsFeasibility StudiesFemaleMaleRatsRats, Sprague-DawleyTenecteplaseTissue Plasminogen ActivatorFibrinolytic AgentsTenecteplaseTissue Plasminogen Activator3 rsAnimal modelCerebral ischemiaRigorThromboemboli

Identifiers

PMID41611917
PMCPMC12855246

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.