Evidence map›Paper›PMID 41611771›Full record

ArticleScientific reports2026

Recombinase polymerase amplification-based rapid detection of Oryctes rhinoceros nudivirus (OrNV) infection in coconut rhinoceros beetles.

Sandeep K Gupta, Nicola K Richards, Tyler Regtien, Mitchell K Weston, Paul H Maclean, Jeanne M E Jacobs, Alasdair D L Noble, Trevor A Jackson, Sean D G Marshall

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sandeep K GuptaAgResearch Group, Bioeconomy Science Institute, Hopkirk Research Institute, Palmerston North, New Zealand. sandeep.gupta@agresearch.co.nz.
Nicola K RichardsAgResearch Group, Bioeconomy Science Institute, Tuhiraki, Lincoln, Christchurch, New Zealand.
Tyler RegtienAgResearch Group, Bioeconomy Science Institute, Hopkirk Research Institute, Palmerston North, New Zealand.
Mitchell K WestonAgResearch Group, Bioeconomy Science Institute, Tuhiraki, Lincoln, Christchurch, New Zealand.
Paul H MacleanAgResearch Group, Bioeconomy Science Institute, Grasslands Research Centre, Palmerston North, New Zealand.
Jeanne M E JacobsAgResearch Group, Bioeconomy Science Institute, Tuhiraki, Lincoln, Christchurch, New Zealand.
Alasdair D L NobleAgResearch Group, Bioeconomy Science Institute, Tuhiraki, Lincoln, Christchurch, New Zealand.
Trevor A JacksonAgResearch Group, Bioeconomy Science Institute, Tuhiraki, Lincoln, Christchurch, New Zealand.
Sean D G MarshallAgResearch Group, Bioeconomy Science Institute, Tuhiraki, Lincoln, Christchurch, New Zealand.

Funding

Ministry of Foreign Affairs and Trade, New Zealand WPG0101699
6 · The paper itself

Abstract

The coconut rhinoceros beetle (CRB) is an important pest of palms in the Asia/Pacific regions. A natural pathogen of CRB, Oryctes rhinoceros nudivirus (OrNV) has successfully been introduced into virus-free, invasive CRB populations in many Pacific islands leading to pest suppression. While PCR is currently used for virus monitoring, a more portable, simplified in-field method of virus detection would be helpful. Here, we developed a Recombinase Polymerase Amplification (RPA)-based assay to detect the partial gp54 gene of OrNV (OrNV-RPA) using fluorescence-based chemistry. The assay has a high degree of specificity with no cross-reactivity to the genomic DNA of various insects, soil, and bacterial species commonly found in the environment. Additionally, the assay has a high level of sensitivity with the ability to detect approximately 1610 copies of the gp54 gene (15 min reaction). OrNV-RPA assay results showed high consistency with the current standard PCR-based assay, whether using purified DNA (95.4%) or crude lysate (93.5%) from CRB midgut tissue, validating the ability of the OrNV-RPA assay to detect virus-free and virus-infected CRB specimens. The newly developed RPA-based assay provides an option for rapid in-field detection of OrNV to help improve CRB management in the Pacific Islands and other regions.

Indexed as

ColeopteraNucleic Acid Amplification TechniquesNudiviridaeRecombinasesAnimalsCocosPolymerase Chain ReactionRapid Diagnostic TestsSensitivity and SpecificityRecombinasesBiological controlEntomopathogenic virusIsothermal amplificationPest managementPortable assay

Identifiers

PMID41611771
PMCPMC12886854

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.