Evidence map›Paper›PMID 41609968›Full record

ArticleEuropean journal of drug metabolism and pharmacokinetics2026

A Validated LC-MS/MS Method for Unbound Rifapentine Quantitation Reveals Nonlinear Plasma Protein Binding and Comparable Free Fractions Between Adults and Children with Tuberculosis.

Wenmei Qiao, Mutong Fang, Miaona Liu, Tian He, Peize Zhang, Wei Li

Abstract readValidation Study
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In one paragraph

Article in European journal of drug metabolism and pharmacokinetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Wenmei QiaoThe Third People's Hospital of Shenzhen, The Second Affiliated Hospital of Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, Guangdong, 518112, People's Republic of China.
Mutong FangThe Third People's Hospital of Shenzhen, The Second Affiliated Hospital of Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, Guangdong, 518112, People's Republic of China.
Miaona LiuThe Third People's Hospital of Shenzhen, The Second Affiliated Hospital of Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, Guangdong, 518112, People's Republic of China.
Tian HeThe Third People's Hospital of Shenzhen, The Second Affiliated Hospital of Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, Guangdong, 518112, People's Republic of China.
Peize ZhangThe Third People's Hospital of Shenzhen, The Second Affiliated Hospital of Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, Guangdong, 518112, People's Republic of China. 82880246@qq.com.
Wei LiThe Third People's Hospital of Shenzhen, The Second Affiliated Hospital of Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, Guangdong, 518112, People's Republic of China. yaojike-01@szsy.sustech.edu.cn.

Funding

Shenzhen fund for Guangdong provincial high-level clinical key specialities SZGSP010Shenzhen High-level Hospital Construction Fund XKJS-2025033Shenzhen High-level Hospital Construction Fund and Shenzhen Clinical Research Center for Tuberculosis 20210617141509001
6 · The paper itself

Abstract

BACKGROUND AND

objectiveTherapeutic drug monitoring (TDM) typically uses total drug concentration (C

methodsA validated LC-MS/MS method used Centrifree ultrafiltration (CF-UF) for free RFPT isolation and isotope internal standard (rifapentine-d8) for quantification. Validation included specificity, linearity (3.00-299.60 ng/mL), accuracy, precision, matrix effects, and stability. Clinical samples from 58 patients with TB (adults and children) receiving RFPT were analyzed. Total and free RFPT, albumin, and biochemical parameters were compared.

resultsThe method showed excellent linearity (R

conclusionsThe LC-MS/MS method is rapid, sensitive, and suitable for routine free RFPT TDM. Nonlinear C

Indexed as

Antibiotics, AntitubercularBlood ProteinsRifampinTuberculosisAdolescentAdultChildChild, PreschoolChromatography, LiquidDrug MonitoringFemaleHumansLiquid Chromatography-Mass SpectrometryMaleMiddle AgedProtein BindingAntibiotics, AntitubercularBlood ProteinsRifampinrifapentine

Identifiers

PMID41609968

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.