Evidence map›Paper›PMID 41609924›Full record

ArticlePhotosynthesis research2026

Preparative isolation and preliminary characterization of LHCII trimers and PSII monomers from Posidonia oceanica L.

Stefano Francesco Farci, Luca Iesu, Domenica Farci, Dario Piano

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Article in Photosynthesis research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Stefano Francesco FarciDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, Italy.
Luca IesuUniversité Paris-Saclay, Univ Evry, CY Cergy Paris Université, CNRS, LAMBE, Cergy, 95000, France.
Domenica FarciDepartment of Plant Physiology, Warsaw University of Life Sciences SGGW, Warsaw, Poland. domenica_farci@sggw.edu.pl.
Dario PianoDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, Italy. dario.piano@unica.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Posidonia oceanica L. is a vascular marine plant that represents a relevant case study in plant physiology due to its phylogenetic position, ecological adaptations, and, in particular, the specialization of its photosynthetic apparatus. Here, we present a procedure for fractional thylakoid solubilization, in which a crude leaf extract obtained by blending is preliminarily depleted of the polyphenolic fraction and subsequently subjected to stepwise solubilization. The resulting thylakoid extracts, processed through a chromatographic workflow, enable the preparative isolation of trimeric Light-Harvesting Complex II and monomeric Photosystem II core complex. Protein identification was supported by mass spectrometry, with peptide homology analysis used for the assignment due to the absence of a curated proteome for P. oceanica. The resulting fractions enable downstream characterization, establishing the first chromatography-based preparative workflow for functional and structural investigations of the photosynthetic apparatus of this ecologically unique species.

Indexed as

AlismatalesLight-Harvesting Protein ComplexesPhotosystem II Protein ComplexMass SpectrometryPhotosynthesisPlant LeavesPlant ProteinsThylakoidsLight-Harvesting Protein ComplexesPhotosystem II Protein ComplexPlant ProteinsAnion exchange chromatographyGene annotationLight harvesting complexMass spectrometryPhotosystem IIPolyphenols extractionPosidonia oceanica L

Identifiers

PMID41609924
PMCPMC12855319

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.