ArticleApplied biochemistry and biotechnology2026
Unveiling the Hydrocarbon-Degrading Potential of Bacillus Cereus BY-16: A Multi-Omics, Proteomics and Biogenic Nanoparticle Profiling-Driven Investigation.
Article in Applied biochemistry and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Petroleum hydrocarbon contamination poses a serious threat to environment and public health due to the persistence of toxic compounds like alkanes, aromatics, and polycyclic aromatic hydrocarbons (PAHs). This research explores isolation and detailed characterization of a novel hydrocarbon-degrading bacterium, Bacillus cereus BY-16, from petroleum-contaminated soil. Among five isolates, BY-16 demonstrated highest hydrocarbon utilization on Bushnell Haas Agar amended with 1% petrol, confirmed through modified DCPIP assays. Unique feature of this study is the integrated application of multi-omics, proteomics and advanced spectroscopic tools. GC-MS and FTIR confirmed the efficient degradation of 105 hydrocarbon compounds into only 29 less-toxic intermediates (e.g., phenol, ethanol, butanoic acid, dihydroxybenzaldehyde and agaricic acid) within 20 days. Raman spectral analysis revealed that BY-16 co-expresses multiple metabolite classes—biosurfactants, siderophores, phenazines, and peptide antibiotics—within the same system. Complementary XRD data identified biogenic FeTiO₃, TiO₂, and chromite nanoparticles, marking the first report of their microbial formation. These nanomaterials, along with biosurfactants, likely enhanced hydrocarbon bioavailability, detoxification, and emulsification. Proteomic profiling (LC-MS/MS) revealed 241 hydrocarbon-responsive proteins, including alkane monooxygenases, cytochrome P450s, ABC transporters, and efflux pumps, showcasing the strain’s metabolic and adaptive versatility. FESEM, EDS and HRTEM analyses further demonstrated biofilm formation, intracellular adaptation, and elemental responses under hydrocarbon stress. This study highlights B. cereus BY-16 as a highly adaptable, nanoparticle-synthesizing bacterium with robust hydrocarbon degradation potential, offering a powerful biological tool for eco-friendly bioremediation of petroleum-contaminated environments. Further experiments in the current study validated the performance of strain BY-16 under field conditions using petroleum contaminated soil.
Indexed as
Identifiers
41609907What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.