Evidence map›Paper›PMID 41608084›Full record

ArticleFrontiers in cell and developmental biology2026

Comparative flow cytometry immunophenotyping of ASCs expanded in conventional flasks versus automated bioreactors.

Hiva Alipour, Guoqiang Ren, Morten Brøndum Sørensen, Sara Aghazadeh, Zongzhe Xuan, Fereshteh Dardmeh, Simone Riis Porsborg, Trine Fink, Qiuyue Peng

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In one paragraph

Article in Frontiers in cell and developmental biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hiva AlipourRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Guoqiang RenThe Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, China.
Morten Brøndum SørensenRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Sara AghazadehRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Zongzhe XuanRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Fereshteh DardmehRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Simone Riis PorsborgRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Trine FinkRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Qiuyue PengRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Adipose-derived stem cells (ASCs) hold significant promises for various regenerative approaches, necessitating the production of a substantial quantity of Methods: ASCs from three donors were cultured and underwent equivalent population doublings in both systems. The cell number was counted to compare the growth rate. Furthermore, the individual expressions of 15 surface markers and their co-expression of 5 (CD73, CD90, CD105, CD166, and CD201) and 8 epitopes (CD34, CD36, CD146, CD248, CD271, CD274, and Stro-1) were analyzed by using multicolor flow cytometry. Results: ASCs expanded in the HFB and TCP system showed a comparable growth rate. Except for a significant downregulation of CD201 in HFB ( Conclusion: The expansion method significantly influences the phenotypic composition of ASCs. HFB systems offer a promising alternative for large-scale ASC manufacturing by better maintaining subpopulation diversity. These findings emphasize the need for functional validation of ASC subtypes and careful consideration of expansion platforms in clinical-grade cell production.

Indexed as

adipose-derived stem cellsheterogeneityimmunophenotypein vitro expansionmultichromatic flow cytometrysubpopulation selection

Identifiers

PMID41608084
PMCPMC12835361

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.