ArticleFrontiers in veterinary science2025
Rapid and specific detection of
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: To develop and validate dual detection platforms integrating recombinase polymerase amplification (RPA) with Pyrococcus furiosus Argonaute (PfAgo) for the rapid and specific identification of Methods: The conserved Results: Two assay formats were established: real-time fluorescence system (RPA-PfAgo-RTF) and lateral flow dipstick system (RPA-PfAgo-LFD). The RPA-PfAgo-RTF assay achieved a detection limit of 10 Conclusion: Both assay formats provide rapid, sensitive, and accurate tools for SS2 detection suitable for laboratory use and on-farm point-of-care testing.
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