ArticleBiomedical reports2026
Evaluation of anionic polymer-coated siRNA lipoplexes prepared using a modified ethanol injection method.
Article in Biomedical reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cationic liposomes are useful carriers for delivering small interfering RNA (siRNA). In our previous study, a simple and efficient modified ethanol injection (MEI) method was developed for the preparation of cationic siRNA lipoplexes. However, non-specific interactions between cationic lipoplexes and biological components, including erythrocytes, induce aggregation, and thus, siRNA is not delivered to the target site after intravenous injection. In the present study, an anionic polymer coating was applied to cationic lipoplexes prepared using the MEI method, and their gene knockdown effect and biodistribution in mice were evaluated. The gene knockdown effect of cationic lipoplexes was preserved after coating with anionic polymers [hyaluronan (HA), chondroitin sulfate (CS) and polyglutamic acid (PGA)], although anionic polymer-coated lipoplexes showed lower cellular association than cationic lipoplexes. Coating of cationic lipoplexes with high-molecular-weight HA, CS and PGA reduced agglutination with erythrocytes. Following intravenous injection, CS- or PGA-coated lipoplexes exhibited lower pulmonary accumulation than cationic lipoplexes, whereas hepatic accumulation of CS- or PGA-coated lipoplexes increased. Collectively, cationic lipoplexes prepared using the MEI method were successfully coated with anionic polymers. Notably, CS or PGA coating of lipoplexes reduced non-specific interactions with erythrocytes, and CS- or PGA-coated lipoplexes could be potential vectors for
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.