Evidence map›Paper›PMID 41607050›Full record

ArticleJournal of integrative plant biology2026

A surrogate-selection-enhanced TnpBmax platform enables efficient genome editing in rice and tomato.

Enze Li, Jiyong Xie, Xinbo Li, Zai Zheng, Mugui Wang, Jian-Kang Zhu, Yifu Tian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of integrative plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Enze LiMinistry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), Institute of Crop Sciences and National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China.ORCID https://orcid.org/0009-0002-4454-5750
Jiyong XieYazhouwan National Laboratory, Sanya, 572024, China.ORCID https://orcid.org/0009-0009-5560-4833
Xinbo LiMinistry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), Institute of Crop Sciences and National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China.ORCID https://orcid.org/0000-0003-1270-9503
Zai ZhengMinistry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), Institute of Crop Sciences and National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China.ORCID https://orcid.org/0000-0001-5773-9402
Mugui WangMinistry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), Institute of Crop Sciences and National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China.ORCID https://orcid.org/0000-0003-0484-086X
Jian-Kang ZhuMinistry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), Institute of Crop Sciences and National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China.ORCID https://orcid.org/0000-0001-5134-731X
Yifu TianMinistry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), Institute of Crop Sciences and National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China.ORCID https://orcid.org/0000-0002-8167-5151

Funding

National Key Research and Development Program of China 2021YFD1201300National Natural Science Foundation of China 32188102National Postdoctoral Program for Innovative Talents BX20220098
6 · The paper itself

Abstract

TnpB, a compact RNA-guided nuclease ancestral to Cas12, is optimized for plant genome editing. A 99-nt enhanced RNA and T5 exonuclease fusion generate TnpBe5, boosting rice editing 2.5-fold. Coupling with a single-strands annealing (SSA)-responsive HYYG surrogate (TnpBmax) enriches edited cells, achieving up to 81.5% efficiency and high homozygosity across rice and tomato.

Indexed as

Gene EditingGenome, PlantOryzaSolanum lycopersicumPlants, Genetically Modifiedgenome editingISDra2riceSSA‐surrogate systemtomato

Identifiers

PMID41607050
PMCPMC13545836

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.