Evidence map›Paper›PMID 41606458›Full record

ArticleBMC microbiology2026

Comprehensive genomic characterization and public health implications of multidrug-resistant Escherichia coli from poultry in Noakhali, Bangladesh.

Nurun Nahar Akter, Md Adnan Munim, Dhirendra Nath Barman, Israt Jahan Asha, Md Murad Hossain, Shuvo Chandra Das, Mohammed Mafizul Islam, Shipan Das Gupta

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Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Nurun Nahar AkterDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Md Adnan MunimDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Dhirendra Nath BarmanDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Israt Jahan AshaDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Md Murad HossainDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Shuvo Chandra DasDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Mohammed Mafizul IslamDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Shipan Das GuptaDepartment of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh. shipan.bge@nstu.edu.bd.

Funding

Noakhali Science and Technology University Research Cell NSTU-RC-BGE-T-23-40 and NSTU-RC-BGE-T-23-41
6 · The paper itself

Abstract

introductionPoultry chickens and their farming environments serve as reservoirs for multi-drug resistant (MDR) pathogenic Escherichia coli (E. coli) that can spread to other species, including humans. In response to this, the study was conducted to assess the antibiotic resistance profile, virulence capabilities, and zoonotic potential of isolates obtained from poultry chickens in Noakhali, Bangladesh.

methodsE. coli isolates were identified through biochemical and molecular methods, including 16S rRNA sequencing. After antibiotic susceptibility testing and multiple antibiotic resistance index (MARI) profiling, two strains out of twenty-four underwent whole-genome Illumina sequencing. For the genetic characterization of these two isolates, genome annotation, serotyping, multilocus sequence typing (MLST), fimbrial typing, plasmid profiling, identification of resistance and virulence genes, detection of mobile genetic elements (MGEs), pan-genome analysis, and phylogenetic comparison were performed.

resultsThe two sequenced strains (one lactose-negative and the other lactose-positive) exhibited resistance to nearly all of the 17 tested antibiotics, with a MAR index of 0.82. Both strains contained ~ 58 AMR genes, including significant genes like blaTEM-1B, qnrS1, aac(3)IId, tet(A), dfrA1, and sul3. Each strain carried six plasmids, including a novel one, along with multiple metal resistance genes (MRGs) that conferred tolerance to arsenic, tellurium, copper, and silver, indicating their ability to survive in metal-rich environments. Pathogenicity prediction suggested a 91.4% probability of causing human infection. Virulence genes related to adhesion, biofilm formation, and immune evasion were identified. Comparative genomics showed similarity to clinical strains.

conclusionAlthough zoonotic transmission was not directly evaluated, genomic similarity and the presence of human-associated virulence and AMR genes suggest a potential public health risk from poultry strains. These findings highlight the zoonotic threat posed by MDR bacteria in poultry farms. Regular monitoring of antibiotic use in poultry, along with genomic surveillance of MDR isolates, is crucial to mitigate this emerging public health concern.

Indexed as

ChickensDrug Resistance, Multiple, BacterialEscherichia coliEscherichia coli InfectionsPoultry DiseasesAnimalsAnti-Bacterial AgentsBangladeshGenome, BacterialGenomicsHumansMicrobial Sensitivity TestsMultilocus Sequence TypingPhylogenyPlasmidsPoultryAnti-Bacterial AgentsRNA, Ribosomal, 16SVirulence FactorsEscherichia coliMultidrug resistancePoultryWhole-genome sequencingZoonotic potential

Identifiers

PMID41606458
PMCPMC12969907

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.