Evidence map›Paper›PMID 41606449›Full record

ArticleJournal of proteome research2026

Molecular Characterization of Calu-3 Cells from Submerged to Air-Liquid Interface to Model Lung Infections.

Deivid Martins Santos, Edmarcia Elisa de Souza, Janaina Macedo-da-Silva, Sueli Mieko Oba-Shinjo, Claudia Blanes Angeli, Vinícius de Morais Gomes, Simon Ngao Mule, Lays Adrianne Mendonça Trajano, Guilherme Antonio de Souza-Silva, Silvia Beatriz Boscardin and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of proteome research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Deivid Martins SantosGlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Edmarcia Elisa de SouzaUnit for Drug Discovery, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Janaina Macedo-da-SilvaGlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Sueli Mieko Oba-ShinjoCellular and Molecular Biology Laboratory, Department of Neurology, Faculty of Medicine (FMUSP), University of São Paulo, São Paulo 01246-903, Brazil.
Claudia Blanes AngeliGlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.ORCID 0000-0002-3906-1973
Vinícius de Morais GomesGlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Simon Ngao MuleGlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Lays Adrianne Mendonça TrajanoGlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Guilherme Antonio de Souza-SilvaLaboratory of Antigen Targeting for Dendritic Cells, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Silvia Beatriz BoscardinLaboratory of Antigen Targeting for Dendritic Cells, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.ORCID 0000-0002-7845-7110
Edison Luiz DurigonLaboratory of Clinical and Molecular Virology, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Ruy Gastaldoni JaegerTumor Microenvironment Lab, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Vanessa Morais FreitasTumor Microenvironment Lab, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Carsten WrengerUnit for Drug Discovery, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.ORCID 0000-0001-5987-1749
Martin Røssel LarsenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M 5230, Denmark.ORCID 0000-0001-6203-0123
Livia Rosa-FernandesGlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.ORCID 0000-0003-1612-1950
Suely Kazue Nagashi MarieCellular and Molecular Biology Laboratory, Department of Neurology, Faculty of Medicine (FMUSP), University of São Paulo, São Paulo 01246-903, Brazil.ORCID 0000-0003-4419-7928
Giuseppe PalmisanoGlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.ORCID 0000-0003-1336-6151

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The air-liquid interface (ALI) model using Calu-3 cells has been used to model lung diseases. In ALI, Calu-3 polarizes and changes to a mucus-producing cell. Polarized Calu-3 similarity with primary cells has been proven; however, no studies have been focusing on the pathways differentially expressed in ALI. Here, we profiled the proteome and transcriptome of Calu-3 from submerged (nonpolarized) to ALI (polarized) conditions, and in the omics data, we observed an increase in cell replication in the nonpolarized condition while polarized cells presented higher activation of cellular energy production, protein maturation and recycle, and expression of immune molecules. Moreover, the omics findings showed upregulation of different biological processes related to the protein quality control system and antigen processing presentation in polarized cells. Immunoblot and fluorescence microscopy confirmed increased expression of bronchial epithelium integrity components such as mucus and tight junctions in polarized cells and revealed a characteristic protein expression and cellular organization found in normal lung epithelium. Furthermore, SARS-CoV-2 infection in polarized cells revealed increased cell death associated with the higher expression of ACE2. The differences observed in this study give us a better understanding of how ALI can mimic human bronchial-epithelial cells and its applications in different contexts of lung diseases.

Indexed as

COVID-19Epithelial CellsProteomeAngiotensin-Converting Enzyme 2Cell LineCell PolarityHumansLungProteomicsSARS-CoV-2Tight JunctionsTranscriptomeACE2 protein, humanAngiotensin-Converting Enzyme 2Proteomeair–liquid interfacecalu-3polarizationproteomicsSARS-CoV-2tight junctionstranscriptomics

Identifiers

PMID41606449
PMCPMC12888003

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.