ArticleThe Science of the total environment2026
Perfluorohexanoic acid (PFHxA) induces cytochrome P450 expression and enzymatic activity in well-differentiated primary human bronchial epithelial (HBE) cells.
Article in The Science of the total environment, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- N-nitrosodimethylamine induces cytochrome P450 activation but not epithelial remodeling in well-differentiated human airway epithelial cells.Cell biology and toxicology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Perfluorohexanoic acid (PFHxA) is a short-chain perfluoroalkyl substance (PFAS) used as a substitute for perfluorooctanoic acid (PFOA) and occurs as a degradation product from long-chain PFAS. PFHxA is found in consumer products and increasingly detected as an indoor dust contaminant suggesting the lung as the target of inhaled PFHxA exposure. Therefore, we aimed to identify potential adverse impacts of PFHxA on primary human bronchial epithelial (HBE) cells, differentiated from basal stem cells in a three-dimensional air-liquid interface culture. This culture forms a pseudostratified epithelium that recapitulates the cellular composition and barrier function of the airway. Using this gold-standard in vitro model of airway cell biology, we performed dose- and time-dependent experiments. Our results indicate that PFHxA exposure up to 1000 nM neither decreases cell viability nor adversely affects epithelial barrier function in HBE cells. However, exposure to 1000 nM PFHxA for 72 h reduced the number of ciliated cells in two donors. Additionally, induction of cytochrome P450 (CYP450) was observed at 24, 48, and 72 h of PFHxA exposure, indicated by increased CYP1A1 and CYP1B1 mRNA expression, CYP1B1 protein expression, and CYP450 enzyme activity. These findings underscore the need for further investigation into PFHxA exposure and its effects on lung health.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.