ArticleMikrochimica acta2026
Photoactivated triple-signal amplification in engineered Pt-Fc-MOFs for rapid and visual detection of foodborne antibiotics.
Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The rational design of a photoactivated platinum–ferrocene-based metal–organic framework (Pt-Fc-MOF) biosensing platform is reported featuring triple-signal amplification for the rapid and visual detection of chloramphenicol (CAP) residues. The system combines aptamer-coated magnetic nanoparticles, cDNA-linked Pt-Fc-MOFs, and light-enhanced catalysis for triple-signal amplification. Upon specific target recognition, CAP binding induces aptamer conformational change, triggering the release of signal probes via nucleic acid strand displacement. The liberated Pt-Fc-MOFs incorporate ferrocene and platinum components that synergistically catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of H₂O₂, which is further enhanced upon light irradiation, generating an intensified colorimetric signal visible to the naked eye. This system demonstrates excellent sensitivity, with a remarkably low limit of detection (LOD) of 0.00016 ng·mL⁻¹, a wide linear detection range spanning from 0.0005 to 10 ng·mL⁻¹ and enables rapid, portable, and minimally instrumented detection. The incorporation of photoactivated catalysis offers a robust and portable strategy for real-time monitoring of foodborne antibiotics, advancing the development of next-generation MOF-based biosensors for point-of-care food safety diagnostics.
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