Evidence map›Paper›PMID 41603470›Full record

ArticleVascular biology (Bristol, England)2026

Comparative analysis of BMP signaling in human ECFCs reveals vascular bed-dependent ALK2 expression.

Nadine Z Großmann, Nurcan Haştar, Yunyun Xiao, Indigo Brakus, Branka Kampfrath, Harm-Jan Bogaard, Robert Szulcek, Reinhold J Medina, Susanne Hildebrandt, Petra Knaus

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Article in Vascular biology (Bristol, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

10 authors.

Nadine Z GroßmannInstitute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.
Nurcan HaştarInstitute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.
Yunyun XiaoInstitute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.
Indigo BrakusInstitute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.
Branka KampfrathInstitute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.
Harm-Jan BogaardDepartment of Pulmonary Medicine, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences, Amsterdam, The Netherlands.
Robert SzulcekLaboratory of In Vitro Modeling Systems of Pulmonary and Thrombotic Diseases, Institute of Physiology, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
Reinhold J MedinaWellcome-Wolfson Institute for Experimental Medicine, Belfast, UK.
Susanne HildebrandtInstitute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.
Petra KnausInstitute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Graphical Abstract: Abstract: Bone morphogenetic protein (BMP) signaling pathway components play a crucial role in cardiovascular homeostasis and sprouting angiogenesis. Endothelial colony-forming cells (ECFCs) are endothelial progenitor cells with a high proliferative and angiogenic capacity and therefore are valuable candidates for angiogenic therapies and vascular regeneration. Of note, a direct comparative analysis of human umbilical cord blood-derived ECFCs (CB-ECFCs) and peripheral blood-derived ECFCs (PB-ECFCs) with a focus on BMP signaling and potential tissue-dependent effects is lacking. In this study, we characterized the BMP signaling responses in ECFCs derived from the umbilical cord and peripheral blood. Analysis of SMAD1/5 phosphorylation showed that BMP2 stimulation only leads to an activation of the BMP signaling pathway in PB-ECFCs, but not in CB-ECFCs. Analysis of gene expression levels of BMP/TGF-β type I and type II receptors demonstrated an elevated expression of ALK2 in PB-ECFCs. Evaluation of sprouting angiogenesis revealed that si-RNA-mediated silencing of ALK2 in CB-ECFCs results in hypersprouting, while si-RNA-mediated silencing of ALK3 leads to hypersprouting in PB-ECFCs. To conclude, we found a differential BMP signaling pathway activation in CB-ECFCs and PB-ECFCs, which is related to vascular bed-dependent expression of ALK2.

Indexed as

ALK2BMP signalingECFCsendothelial progenitor cellsvascular bed

Identifiers

PMID41603470
PMCPMC12899316

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.