Functional assessment of local versus systemic adipose-derived stromal cell therapy in rodent peripheral nerve regeneration.

Introduction: Adipose-derived stromal cell (ASC)-based therapies may play an important role in peripheral nerve regeneration, though the optimal route of application remains unclear. Systemically injected ASCs have been recognized to home to sites of inflammation and injury but also facilitate a widespread regenerative effect throughout the body. The advantage of local ASC administration is the direct and concentrated application at the site of need. Here, we investigated the effect of local versus systemic ASCs on functional recovery in a rodent sciatic nerve injury model. Methods: Twenty Lewis rats underwent sciatic transection and repair with a 10 mm nerve autograft and were subsequently assigned into three groups based on the ASC-treatment: systemic (SYS; Results: The swim test analyses noticed the nerve lesion and assessed functional recovery. Horizontal hindlimb excursion improved slightly and showed earlier recovery in the SYS group compared to LOC at week 6 ( Conclusion: Current ASC treatments modestly enhance functional recovery and reduce muscular atrophy after peripheral nerve injury. A slight trend toward better therapeutic effect was observed with systemic administration, although the differences remained small compared to local application. Ideal dosage, optimal timepoint as well as combination of local and systemic ASC-therapies need further assessment.