Comparative Analysis of the Oviducts in Wanyue Black Pigs with Different Parities Based on RNA-Seq.

Hanyu Zhou, Huibin Zhang, Ping Wu, Fang Tian, Jinyu Guan, Yifan Sun, Xiaodong Zhang, Zongjun Yin

Abstract read

In one paragraph

Article in Veterinary sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Hanyu ZhouAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

Huibin ZhangAnhui Key Laboratory of Livestock and Poultry Product Safety, Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China.ORCID 0000-0002-4626-1236

Ping WuAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

Fang TianAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

Jinyu GuanAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

Yifan SunAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

Xiaodong ZhangAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

Zongjun YinAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.ORCID 0000-0001-9893-743X

Funding

The Biological Breeding-Major Projects in National Science and Technology 2023ZD0404402-03The Joint Research Project on Local Pig Breeding in Anhui Province 340000211260001000431

6 · The paper itself

Abstract

This study investigates the molecular mechanisms underlying parity's impact on sow reproductive function by comparing the transcriptome profiles of high-parity (9 parities) and low-parity (1 parity) Wanyue Black pigs. Oviduct tissues were collected and subjected to RNA-seq analysis. Differentially expressed genes (DEGs) were identified using the DESeq2 algorithm. A total of 4218 DEGs were detected, with 2421 up-regulated and 1797 down-regulated genes. Functional enrichment analysis using GO and KEGG revealed that these DEGs were significantly associated with reproductive pathways, including cilium movement, oocyte maturation, and steroid hormone biosynthesis. Protein-protein interaction (PPI) network analysis highlighted key genes such as HSD3B1 and DNAI1, which play central roles in the parity differences. The expression patterns of selected candidate genes were further validated by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), and the results were consistent with the RNA-seq data, confirming the reliability of our findings. This study provides valuable insights into the molecular regulation of parity in sows and offers a theoretical basis for optimizing reproductive management and breeding strategies in pig production.

Indexed as

oviductsparityPPI network analysisRNA-seqWanyue black pig

Identifiers

PMID41600680

DOI10.3390/vetsci13010024

PMCPMC12846499

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.