Highly Sensitive Hybridization Chain Reaction-Based miRNA Detection Technology Using Diffusivity Analysis of Fluorescent Probe-Modified miRNA Particles.

Momoka Nakai, Yui Watanabe, Maho Koda, Chisato Sakamoto, Tatsuhito Hasegawa, Han-Sheng Chuang, Hiroaki Sakamoto

Abstract read

In one paragraph

Article in Sensors (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

From In Silico to Clinic: Harnessing Noncoding RNAs for Diabetes Research and Therapy, a Step toward ncRNA Precision.ACS pharmacology & translational science · 2026
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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Momoka NakaiDepartment of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Fukui, Japan.

Yui WatanabeDepartment of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Fukui, Japan.

Maho KodaDepartment of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Fukui, Japan.

Chisato SakamotoChemistry and Biology, National Institute of Technology, Fukui College, Sabae 916-0064, Fukui, Japan.

Tatsuhito HasegawaFundamental Engineering for Knowledge-Based Society, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Fukui, Japan.ORCID 0000-0002-0768-1406

Han-Sheng ChuangDepartment of Biomedical Engineering, National Cheng Kung University, Tainan 701401, Taiwan.ORCID 0000-0002-7257-6565

Hiroaki SakamotoDepartment of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Fukui, Japan.ORCID 0000-0002-6300-4055

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) are promising biomarkers for the early detection of various diseases, particularly cancer, driving active development of highly sensitive and selective detection technologies. This study aims to establish a novel miRNA detection technique that utilizes image analysis to track the Brownian motion (diffusivity) of fluorescent probe-modified miRNA particles. This method identifies the presence and concentration of miRNAs by exploiting the change in particle size upon hybridization with the target. Furthermore, the use of a probe modified with a photo-crosslinkable artificial nucleic acid (CNV-D) enables the covalent capture of the target miRNA, ensuring high selectivity in biological samples even under stringent washing conditions. By integrating Hybridization Chain Reaction (HCR), the complex size is significantly amplified, dramatically enhancing the detection sensitivity. Consequently, we successfully demonstrated the highly sensitive and specific detection of the cancer biomarker miR-21 in serum, achieving an exceptionally low limit of detection (LOD) of 1 fM. This technology holds great potential to contribute to the early diagnosis of cancer.

Indexed as

Biosensing TechniquesFluorescent DyesMicroRNAsNucleic Acid HybridizationBiomarkers, TumorHumansLimit of DetectionBiomarkers, TumorFluorescent DyesMicroRNAsMIRN21 microRNA, humanhybridization chain reactionimage analysisMicroRNAphoto-crosslinkable artificial nucleic acidprobe-modified particle

Identifiers

PMID41600515

DOI10.3390/s26020713

PMCPMC12845866

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.