Design and Validation of a Pressure-Driven Liquid Metering System with Heated PTFE Tubing for Laboratory Automation.

This paper presents a pressure-driven liquid transfer system for laboratory automation, along with a physics-based model and calibration method. The device maintains near-isothermal transport by storing reagents at a prescribed temperature and routing the flow through a single PTFE tube enclosed within a heated jacket. The pressure-drop model accounts for temperature-dependent viscosity and the thermal expansion of PTFE. Residual deviations from the no-slip prediction in submillimeter tubing are represented by an effective slip length, which is identified through linear regression. This parameter is subsequently used to calculate the pressure required to achieve a target flow rate. Experimental results compare unheated and heated operating conditions and characterize the dependence of slip length on temperature and flow rate. Under heated operation with slip-compensated pressure commands, the system achieved dispensing accuracy within ±4% over the tested range, whereas unheated operation exhibited larger errors due to axial temperature gradients. These results demonstrate that effective thermal management and slip compensation are critical for accurate pressure-based metering under temperature-sensitive conditions, as validated using water-based tests.