Development of a Fluorophore-Bound l-Tryptophan Derivative for Evaluating Indoleamine 2,3-Dioxygenase Activity by HPLC with Fluorescence Detection: An In Vivo Microdialysis Study Using Rat Kidney.

Mayu Onozato, Reika Aoki, Mai Yamaguchi, Honoka Fujimoto, Tatsuya Sakamoto, Takeshi Fukushima

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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0 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mayu OnozatoFaculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi 274-8510, Chiba, Japan.

Reika AokiFaculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi 274-8510, Chiba, Japan.

Mai YamaguchiFaculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi 274-8510, Chiba, Japan.

Honoka FujimotoFaculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi 274-8510, Chiba, Japan.

Tatsuya SakamotoFaculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi 274-8510, Chiba, Japan.

Takeshi FukushimaFaculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi 274-8510, Chiba, Japan.ORCID 0000-0002-6086-4156

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Evaluating the activity of indoleamine 2,3-dioxygenase (IDO), the rate-limiting enzyme in tryptophan (Trp) metabolism, is important because IDO is involved in immune tolerance and drives the production of Trp metabolites implicated in psychiatric disorders and cancer. This study aimed to design and develop a novel fluorescent l-Trp derivative to fluorometrically monitor Trp-catabolizing enzyme activity via IDO. To evaluate IDO activity in vivo, 7-

Indexed as

Fluorescent DyesIndoleamine-Pyrrole 2,3,-DioxygenaseKidneyMicrodialysisTryptophanAnimalsChromatography, High Pressure LiquidFluorescenceMaleRatsRats, Sprague-DawleyFluorescent DyesIndoleamine-Pyrrole 2,3,-DioxygenaseTryptophanfluorescenceHPLCindoleamine 2,3-dioxygenasemicrodialysisrat kidney

Identifiers

PMID41599332

DOI10.3390/molecules31020283

PMCPMC12843689

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.