Effects of Microgravity, Hypergravity, and Ionizing Radiation on the Enzymatic Activity of Proteinase K.

Bartosz Rybacki, Wojciech Wysocki, Tomasz Zajkowski, Robert Brodzik, Beata Krawczyk

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bartosz RybackiDepartment of Biotechnology and Microbiology, Faculty of Chemistry, Gdansk University of Technology, 80-233 Gdansk, Poland.ORCID 0000-0002-2776-5439

Wojciech WysockiPolish Astrobiology Society, 02-697 Warsaw, Poland.

Tomasz ZajkowskiPolish Astrobiology Society, 02-697 Warsaw, Poland.ORCID 0000-0001-9433-6071

Robert BrodzikResearch and Development Department, QIAGEN Gdansk (Blirt SA), 80-172 Gdansk, Poland.

Beata KrawczykDepartment of Biotechnology and Microbiology, Faculty of Chemistry, Gdansk University of Technology, 80-233 Gdansk, Poland.ORCID 0000-0001-5528-8898

Funding

Ministry of Education and Science, Poland MEiN/2022/DIR/3437Ministry of Science and Higher Education MEiN/2021/234/DIR/NN4RADIUM LEARNING THROUGH RESEARCH PROGRAMS DEC-14/2/2023/IDUB/III.1a/Ra/2023

6 · The paper itself

Abstract

Space conditions offer new insights into fundamental biological and molecular mechanisms. The study aimed to evaluate the enzymatic activity of proteinase K (PK) under extreme conditions relevant to space environments: simulated microgravity, hypergravity, and gamma radiation. PK activity was tested using azocasein (AZO) as a chromogenic substrate, with enzymatic reactions monitored spectrophotometrically at 450 nm. A rotating wall vessel (RWV) simulated microgravity, centrifugation at 1000×

Indexed as

Endopeptidase KHypergravityRadiation, IonizingWeightlessnessGamma RaysEndopeptidase Kenzymatic activitygamma radiationhypergravitymicrogravitymolecular biotechnologyproteinase Kspace biology

Identifiers

PMID41599278

DOI10.3390/molecules31020229

PMCPMC12843933

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.