ArticlePathogens (Basel, Switzerland)2026
Molecular and Culture-Based Surveillance of Free-Living Amoebae in Human Related Sources in an Outermost Region.
Marco D Peña-Prunell, María Reyes-Batlle, Patricia Pérez-Pérez, Rubén L Rodríguez-Expósito, Ines Sifaoui, Omar García-Pérez, Angélica T Domínguez-de Barros, Elizabeth Córdoba-Lanús, José E Piñero, Jacob Lorenzo-Morales
Abstract read
In one paragraphArticle in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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0citing papers in PubMed
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What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
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4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
10 authors.
Marco D Peña-PrunellInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0009-0000-1428-7602 María Reyes-BatlleInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0000-0002-2290-5746 Patricia Pérez-PérezInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0000-0002-7719-9164 Rubén L Rodríguez-ExpósitoInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0000-0002-3858-836X Ines SifaouiInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0000-0002-5649-8333 Omar García-PérezInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0000-0001-6149-5949 Angélica T Domínguez-de BarrosInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0009-0008-8562-6403 Elizabeth Córdoba-LanúsInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0000-0001-5534-8280 José E PiñeroInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0000-0001-6233-8224 Jacob Lorenzo-MoralesInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Av. Astrofísico Francisco Sanchez, s/n, 38200 San Cristóbal de La Laguna, Spain.ORCID 0000-0002-7683-2888 Funding
Cabildo Insular de Tenerife CC20230222, CABILDO.23CIBERINFEC CB21/13/00100MePRAM Project PMP22/00092Plan Propio de Investigación 2025, Universidad de La Laguna 2025/0002183PROMETEO Program, Programa de Ayudas para Grupos de Investigación de Excelencia CIPROM/2024/88
6 · The paper itselfAbstract
In this study, we investigated the presence and diversity of FLA in 62 environmental samples collected across Tenerife, Canary Islands, Spain including agricultural and playground soils, and on double treated water from public refrigerated fountains. Amoebae were isolated by culturing processed samples onto 2% Non-Nutrient Agar plates (NNA) which were checked daily for further processing up to molecular characterization. In this case, two approaches for molecular identification were assessed: direct multiplex qPCR targeting four potentially pathogenic FLA (
Indexed as
AmoebaBalamuthia mandrillarisHumansSpainagricultural soilfree living amoebaplayground soilpublic refrigerated fountainstolerance assays
Identifiers
PMID41599058
PMCPMC12844781
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