Comparative Transcriptomics Reveals Novel and Differential Long-Noncoding RNA Responses Underlying Interferon-Mediated Antiviral Regulation in Porcine Alveolar Macrophages.

Jiuyi Li, Oluwaseun Adeyemi, Laura C Miller, Yongming Sang

Abstract readComparative Study

In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Domestication-Driven Expansion and Structural Convergence of the Porcine Antiviral Interferon Repertoire.Biomolecules · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jiuyi LiDepartment of Food and Animal Sciences, College of Agriculture, Tennessee State University, Nashville, TN 37209, USA.ORCID 0009-0003-3649-7100

Oluwaseun AdeyemiDepartment of Food and Animal Sciences, College of Agriculture, Tennessee State University, Nashville, TN 37209, USA.

Laura C MillerDiagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave., Manhattan, KS 66506, USA.ORCID 0000-0002-8946-9416

Yongming SangDepartment of Food and Animal Sciences, College of Agriculture, Tennessee State University, Nashville, TN 37209, USA.ORCID 0000-0002-2980-2854

Funding

NSF IOS-1831988United States Department of Agriculture Evans-Allen-1013186United States Department of Agriculture NIFA AFRI 2020-67016-31347United States Department of Agriculture NIFA AFRI 2025-38821-45500

6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major threat to the global swine industry. Long non-coding RNAs (lncRNAs) are emerging as crucial regulators of antiviral immunity, but their roles in porcine alveolar macrophages (PAMs)-the primary target of PRRSV-remain poorly characterized. This study presents a genome-wide analysis of lncRNA expression in PAMs stimulated with a PRRS modified live virus (MLV) vaccine and two type I interferons, IFN-α1 and IFN-ω5. Whole-transcriptome sequencing identified over 2000 differentially expressed lncRNAs, with IFN-ω5 inducing the most extensive transcriptional reprogramming. Weighted gene co-expression network analysis (WGCNA) revealed interferon-specific lncRNA-mRNA modules, and functional enrichment showed these lncRNAs are involved in key immune and metabolic pathways, including chemokine signaling, MAPK, and mTOR. Our findings establish a comprehensive landscape of lncRNA regulation in PAMs, highlighting their role in fine-tuning the antiviral responses and suggesting novel targets for interferon-based antiviral interventions against PRRSV.

Indexed as

InterferonsMacrophages, AlveolarPorcine respiratory and reproductive syndrome virusRNA, Long NoncodingTranscriptomeAnimalsGene Expression ProfilingGene Expression RegulationPorcine Reproductive and Respiratory SyndromeSignal TransductionSwineInterferonsRNA, Long Noncodingalveolar macrophageinterferonlong non-coding RNAporcine reproductive and respiratory syndromePRRS virus

Identifiers

PMID41599019

DOI10.3390/pathogens15010035

PMCPMC12844857

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.