Evidence map›Paper›PMID 41597736›Full record

ArticleMicroorganisms2026

Quantitative Assessment of Total Aerobic Viable Counts in Apitoxin-, Royal-Jelly-, Propolis-, Honey-, and Bee-Pollen-Based Products Through an Automated Growth-Based System.

Harold A Prada-Ramírez, Raquel Gómez-Pliego, Humberto Zardo, Willy-Fernando Cely-Veloza, Ericsson Coy-Barrera, Rodrigo Palacio-Beltrán, Romel Peña-Romero, Sandra Gonzalez-Alarcon, Juan Camilo Fonseca-Acevedo, Juan Pablo Montes-Tamara and 5 more

Abstract read
In one paragraph

Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Harold A Prada-RamírezLaboratorios Coaspharma S.A.S., CL 18A 28A-43, Bogotá 111411, Colombia.ORCID 0000-0003-3490-5329
Raquel Gómez-PliegoDepartamento de Ciencias Biológicas, Sección de Ciencias de la Salud Humana, Facultad de Estudios Superiores Cuautitlán-UNAM, Cuautitlán Izcalli 54740, Estado de México, Mexico.ORCID 0000-0002-6227-0511
Humberto ZardoSchool of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, SP, Brazil.
Willy-Fernando Cely-VelozaUnidad de Investigación Básica Oral-UIBO, Vicerrectoría de Investigaciones, Facultad de Odontología, Universidad El Bosque, Bogotá 111321, Colombia.
Ericsson Coy-BarreraLaboratorio de Química Bioorgánica, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cajicá 250247, Colombia.ORCID 0000-0002-3553-9749
Rodrigo Palacio-BeltránLaboratorios Coaspharma S.A.S., CL 18A 28A-43, Bogotá 111411, Colombia.
Romel Peña-RomeroLaboratorios Coaspharma S.A.S., CL 18A 28A-43, Bogotá 111411, Colombia.
Sandra Gonzalez-AlarconLaboratorios Coaspharma S.A.S., CL 18A 28A-43, Bogotá 111411, Colombia.
Juan Camilo Fonseca-AcevedoLaboratorios Coaspharma S.A.S., CL 18A 28A-43, Bogotá 111411, Colombia.
Juan Pablo Montes-TamaraLaboratorios Coaspharma S.A.S., CL 18A 28A-43, Bogotá 111411, Colombia.
Lina Nieto-CelisLaboratorios Coaspharma S.A.S., CL 18A 28A-43, Bogotá 111411, Colombia.
Ruth Dallos-AcostaNeogen Colombia S.A.S., Bogotá 0810, Colombia.
Tatiana GonzalezNeogen Colombia S.A.S., Bogotá 0810, Colombia.
David Díaz-BáezUnidad de Investigación Básica Oral-UIBO, Vicerrectoría de Investigaciones, Facultad de Odontología, Universidad El Bosque, Bogotá 111321, Colombia.ORCID 0000-0001-8890-6250
Gloria Inés LafaurieUnidad de Investigación Básica Oral-UIBO, Vicerrectoría de Investigaciones, Facultad de Odontología, Universidad El Bosque, Bogotá 111321, Colombia.ORCID 0000-0003-3986-0625

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bee-derived products such as apitoxin, royal jelly, propolis, bee pollen, and honey are increasingly being used as part of cosmetic products because all of them contain a large number of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial, and regenerative properties, which enable them to be used for therapeutic purposes. The aim of this investigation was to assess the performance of an automated growth-based system in order to make a quantitative examination of the total aerobic viable counts in bee-derived personal care products using NF-TVC vials that contained a nutrient-based medium with dextrose as the carbon source. According to USP general chapter <1223>, pivotal validation criteria such as linearity, equivalence of results, operative range, precision, accuracy, ruggedness, limit of quantification, and limit of detection have demonstrated that the automated system can be used for a reliable total aerobic viable count. Moreover, the actual research demonstrated that polysorbates efficiently block the antimicrobiological potential of bioactive compounds, such as phenols, flavonoids, enzymes, peptides, and fatty acids, which naturally occur in apitoxin, royal jelly, propolis, bee pollen, and honey, allowing for efficient microorganism recovery from the bee-made products tested. Therefore, this AGBS could be applied efficiently within the cosmetic industry to assess the total aerobic viable count in bee-derived products such as capillary treatments, toothpaste, and anti-aging cream, affording several benefits associated with faster product release into the market.

Indexed as

apitoxinautomated growth-based systembacteriabee pollenhoneymoldspropolisroyal jellyyeast

Identifiers

PMID41597736
PMCPMC12843669

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.