Evidence map›Paper›PMID 41596811›Full record

ArticleFoods (Basel, Switzerland)2026

Multidimensional Analysis Reveals the Flavor Quality Formation Mechanism During the Primary Pile Fermentation of Dark Tea.

Dunchao Wu, Yufei He, Juanshu Wen, Hongfa Zheng, Xi Zhao, Penghui Yu, Ni Zhong, Li Niu, Shi Li, Yong Lin and 2 more

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Dunchao WuKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China.
Yufei HeTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Juanshu WenKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China.
Hongfa ZhengTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Xi ZhaoTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Penghui YuTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.ORCID 0000-0002-4852-2209
Ni ZhongTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Li NiuKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China.
Shi LiKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China.
Yong LinKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China.
Hao HuangTea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.ORCID 0000-0002-8002-1236
Zhonghua LiuKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China.ORCID 0000-0003-0000-4565

Funding

Hunan Provincial Natural Science Foundation 2024JJ5216National Key R&D Program of China 2022YFD1600804National Tea Industry Technology System Project of China CARS-19Research and Development Program in Key Areas of Hunan Province 2023NK2025
6 · The paper itself

Abstract

Pile fermentation is a crucial process for developing the characteristic mellow taste and aged aroma of dark tea, yet the internal quality transformation mechanism of this process is still unclear. This study employed a high-sensitivity analytical platform based on gas chromatography-mass spectrometry (GC-MS) to systematically investigate the dynamic interplay between key chemical components, enzyme activities, and volatile compounds during the pile fermentation of primary dark tea. Our findings revealed a significant decrease in ester-type catechins, crude protein, and protopectin, alongside a notable accumulation of non-ester-type catechins, gallic acid, and soluble components. The multi-enzyme system-comprising PPO/POD, pectinase/cellulase, and protease-cooperatively drove the oxidation of phenols, cell wall degradation, and the release of aromatic precursors. This was complemented by GC-MS analysis, which identified and quantified 103 volatile compounds across nine chemical classes. The total content of volatile compounds increased significantly, with alcohols, esters, and aldehydes/ketones being the dominant groups. Floral and fruity compounds such as linalool and geraniol accumulated continuously, while esters exhibited an initial increase followed by a decrease. Notably, carotenoid degradation products, including β-ionone, were significantly enriched during the later stages. This study revealed a "oxidation-hydrolysis-reconstruction" metabolic mechanism co-driven by microbial activity and a multi-enzyme system, providing a theoretical foundation for the precise regulation of pile fermentation and targeted quality improvement of primary dark tea.

Indexed as

GC-MSmulti-enzymes synergistic effectpile fermentationvolatile compounds

Identifiers

PMID41596811
PMCPMC12840366

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.