Evidence map›Paper›PMID 41596715›Full record

ReviewInternational journal of molecular sciences2026

Molecular Identification and RNA-Based Management of Fungal Plant Pathogens: From PCR to CRISPR/Cas9.

Rizwan Ali Ansari, Younes Rezaee Danesh, Ivana Castello, Alessandro Vitale

Abstract readReview
In one paragraph

Review in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rizwan Ali AnsariDepartment of Ecology and Life Safety, Faculty of Geography and Ecology, Samarkand State University Named After Sharof Rashidov, Samarkand 140104, Uzbekistan.ORCID 0000-0003-1632-4627
Younes Rezaee DaneshDepartment of Plant Protection, Faculty of Agriculture, Van Yuzuncu Yil University, Van 65090, Türkiye.ORCID 0000-0003-1060-0264
Ivana CastelloDepartment of Agriculture, Food and Environment, University of Catania, Via S. Sofia 100, 95123 Catania, Italy.
Alessandro VitaleDepartment of Agriculture, Food and Environment, University of Catania, Via S. Sofia 100, 95123 Catania, Italy.ORCID 0000-0002-7339-4034

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fungal diseases continue to limit global crop production and drive major economic losses. Conventional diagnostic and control approaches depend on time-consuming culture-based methods and broad-spectrum chemicals, which offer limited precision. Advances in molecular identification have changed this landscape. PCR, qPCR, LAMP, sequencing and portable platforms enable rapid and species-level detection directly from plant tissue. These tools feed into RNA-based control strategies, where knowledge of pathogen genomes and sRNA exchange enables targeted suppression of essential fungal genes. Host-induced and spray-induced gene silencing provide selective control without the long-term environmental costs associated with chemical use. CRISPR/Cas9 based tools now refine both diagnostics and resistance development, and bioinformatics improves target gene selection. Rising integration of artificial intelligence indicates a future in which disease detection, prediction and management connect in near real time. The major challenge lies in limited field validation and the narrow range of fungal species with complete molecular datasets, yet coordinated multi-site trials and expansion of annotated genomic resources can enable wider implementation. The combined use of molecular diagnostics and RNA-based strategies marks a shift from disease reaction to disease prevention and moves crop protection towards a precise, sustainable and responsive management system. This review synthesizes the information related to current molecular identification tools and RNA-based management strategies, and evaluates how their integration supports precise and sustainable approaches for fungal disease control under diverse environmental settings.

Indexed as

CRISPR-Cas SystemsFungiPlant DiseasesRNA, FungalGenome, FungalPlantsPolymerase Chain ReactionRNA, Fungalbiosafetydiagnosisdisease managementplant healthRNAi

Identifiers

PMID41596715
PMCPMC12842041

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.