Evidence map›Paper›PMID 41596700›Full record

ArticleInternational journal of molecular sciences2026

Insights into the Performance of CusF as a Solubility Tag for Recombinant Protein Expression.

Igor P Oscorbin, Maria A Smertina, Maria S Kunova, Maxim L Filipenko

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Igor P OscorbinInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), 8, Lavrentiev Avenue, Novosibirsk 630090, Russia.ORCID 0000-0003-3754-5824
Maria A SmertinaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), 8, Lavrentiev Avenue, Novosibirsk 630090, Russia.
Maria S KunovaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), 8, Lavrentiev Avenue, Novosibirsk 630090, Russia.
Maxim L FilipenkoInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (ICBFM SB RAS), 8, Lavrentiev Avenue, Novosibirsk 630090, Russia.ORCID 0000-0002-8950-5368

Funding

Russian Science Foundation 24-24-00389
6 · The paper itself

Abstract

The metal-binding periplasmic protein CusF has been proposed as a bifunctional tag that enhances the solubility of recombinant proteins and enables purification using Cu affinity chromatography. However, evidence for its performance remains limited to a few model proteins. Here, we evaluated CusF as a solubility tag for two heterologous proteins: a putative poly(A)-polymerase from

Indexed as

Copper Transport ProteinsEscherichia coli ProteinsRecombinant Fusion ProteinsChromatography, AffinityCopperEnterococcus faecalisEscherichia coliLuminescent ProteinsRecombinant ProteinsRed Fluorescent ProteinSolubilityCopperCopper Transport ProteinsCusF protein, E coliEscherichia coli ProteinsLuminescent ProteinsRecombinant Fusion ProteinsRecombinant ProteinsRed Fluorescent ProteinCusFEnterococcus faecalisfluorescent proteinfusion proteininclusion bodiesmCherryPAPpoly(A)-polymeraseprotein expression

Identifiers

PMID41596700
PMCPMC12842202

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.