Evidence map›Paper›PMID 41596535›Full record

ArticleInternational journal of molecular sciences2026

Reference Gene Stability in

Monika Bielecka, Bartosz Pencakowski, Marta Stafiniak, Weronika Kozłowska, Michał Dziwak, Katarzyna Nowis, Łukasz Łaczmański, Adam Matkowski

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Monika BieleckaDepartment of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland.ORCID 0000-0002-0067-6073
Bartosz PencakowskiDepartment of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland.
Marta StafiniakDepartment of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland.ORCID 0000-0002-8315-7569
Weronika KozłowskaDepartment of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland.ORCID 0000-0001-8330-4576
Michał DziwakDepartment of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland.
Katarzyna NowisLaboratory of Genomics & Bioinformatics, Hirszfeld Institute of Immunology and Experimental Therapy PAS, 53-114 Wroclaw, Poland.ORCID 0000-0002-0415-0271
Łukasz ŁaczmańskiLaboratory of Genomics & Bioinformatics, Hirszfeld Institute of Immunology and Experimental Therapy PAS, 53-114 Wroclaw, Poland.ORCID 0000-0002-0874-5483
Adam MatkowskiDepartment of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland.ORCID 0000-0002-7283-2653

Funding

Ministry of Science and Higher Education 28/598769/SPUB/SP/2024National Science Centre OPUS 2020/39/I/NZ7/01515
6 · The paper itself

Abstract

Quantitative real-time PCR (qPCR) remains a cornerstone method for analyzing gene expression due to its high sensitivity, specificity, and reproducibility. However, for reliable results in relative quantification studies, the choice of an appropriate reference gene is critical to ensure accurate normalization. The expression of commonly used reference genes can vary depending on developmental stage and experimental conditions, making their validation essential. To date, no validated reference genes have been reported for

Indexed as

Genes, PlantReal-Time Polymerase Chain ReactionGene Expression ProfilingGene Expression Regulation, PlantGenes, EssentialReference StandardsReproducibility of ResultsAgrostemma githagohousekeeping genesqRT-PCR normalizationreference-gene validationRefFinder

Identifiers

PMID41596535
PMCPMC12840974

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.