ArticleBioengineering (Basel, Switzerland)2025
Engineering Resilience: How Irradiation Strategies Influence 3D-Bioprinted Adipose Stem Cells.
Article in Bioengineering (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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10 authors.
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Abstract
backgroundReconstructive defect coverage after irradiation remains a challenge in reconstructive surgery, as ionizing radiation leads to tissue ischemia and fibrosis. Therefore, the application of adipose-derived stem cells (ASCs) might be a therapeutic strategy for improving flap survival. Nevertheless, the influence of irradiation on ASCs remains unclear. This study examines the effect of irradiation on 3D-printed ASCs.
methodsASCs were 3D-cultured. The constructs were irradiated with 2 Gy and 5 Gy; one group treated with 0 Gy served as the non-irradiated control group. Cell viability was measured via a WST-8 assay, a live/dead assay and fluorescence microscopy 24 h, 48 h and 72 h after irradiation. Furthermore, qPCR analysis was performed to detect the expression of HIF-1α, p53 and IL-6 at the same timepoints.
resultsCell survival was high after 24 h. Expression of HIF1α after 24 h was 1.5 times significantly higher in the 2 Gy group compared with the 0 Gy group. The expression of other genes was not significantly affected by irradiation. Measurement of the metabolic activity and survival of the ASCs did not show differences between the different groups at all timepoints.
conclusions3D-cultured adipose-derived stem cells maintain high viability after moderate irradiation, suggesting radioresistance.
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