Evidence map›Paper›PMID 41593495›Full record

ArticleBMC plant biology2026

Identification and functional characterization of a novel gene conferring root rot resistance in Panax Notoginseng.

Manqiao Li, Zihan Yang, Kuixiu Li, Xuyan Liu, Yueyue Zhu, Zihan Yang, Fugang Wei, Shengchao Yang, Guanze Liu

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Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Manqiao LiCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Zihan YangCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Kuixiu LiCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Xuyan LiuCollege of Plant Protection, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Yueyue ZhuCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Zihan YangThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National & Local Joint Engineering Research Center on Germplasms Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, 650201, China.
Fugang WeiWenshan Miaoxiang Notoginseng Industry Co., Ltd, Wenshan, Yunnan, 663000, China.
Shengchao YangYunnan Seed Laboratory, Yunnan Agricultural University, Kunming, 650201, China. yangshengchao@uoh.edu.cn.
Guanze LiuThe Key Laboratory of Medicinal Plant Biology of Yunnan Province, National & Local Joint Engineering Research Center on Germplasms Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, 650201, China. guanzeliu@ynau.edu.cn.

Funding

Major Special Science and Technology Project of Yunnan Province 202502AU100003-1National Natural Science Foundation of China 32260095Young Talent Project of the "Xing Dian Talent Support Program" of Yunnan Province in 2022 XDYC-QNRC-2022-0210Yunnan Seed Laboratory 202205AR070001Yunnan Sino-Vietnam Joint International Research Laboratory for Rare Panax Resources 202503AP140007
6 · The paper itself

Abstract

backgroundPanax notoginseng is highly susceptible to root rot during cultivation, a disease that severely reduces yield and compromises quality, thereby constraining sustainable industry development. To identify candidate genes for root rot resistance, we compared diseased and healthy plants using an integrated RNA-seq and BSA-seq approach.

resultsRNA-seq analysis identified 3,022 significantly differentially expressed genes, with 1,399 upregulated and 1,623 downregulated in diseased tissues. Integration with BSA-seq data pinpointed eight candidate resistance genes. Among these, the receptor-like cytoplasmic kinase gene PnRLCK1 showed pronounced induction in samples infected with root rot. Functional studies revealed that RNA interference-mediated silencing of PnRLCK1 significantly compromised resistance to Fusarium oxysporum, while its transient overexpression in Nicotiana benthamiana potentiated local defense responses.

conclusionUsing an integrated RNA-seq and BSA-seq approach, this study identified an RLCK gene, PnRLCK1, associated with root rot resistance in P. notoginseng, thereby contributing to a better understanding of the molecular basis of root rot resistance and providing genetic resources for disease-resistant breeding.

Indexed as

Disease ResistanceFusariumGenes, PlantPanax notoginsengPlant DiseasesPlant ProteinsPlant RootsGene Expression Regulation, PlantPlant ProteinsBSA-seqRLCKRNAiRNA-seqRoot rot

Identifiers

PMID41593495
PMCPMC12918092

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