Evidence map›Paper›PMID 41593368›Full record

ArticleAnalytical and bioanalytical chemistry2026

Neutralizing activity analysis of mimotopes against porcine epidemic diarrhea virus (PEDV) spike protein.

Tae-Hun Kim, Jae-Yeon Park, Soonil Kwon, Jaeyong Jung, Jeong Soo Sung, Dong-Ho Han, Min-Jung Kang, Joachim Jose, Hyun-Jin Shin, Jae-Chul Pyun

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tae-Hun KimDepartment of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
Jae-Yeon ParkCollege of Veterinary Medicine, Chungnam National University, Daejeon, 34134, Republic of Korea.
Soonil KwonDepartment of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
Jaeyong JungDepartment of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
Jeong Soo SungDepartment of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
Dong-Ho HanDepartment of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
Min-Jung KangKorea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea.
Joachim JoseInstitute of Pharmaceutical and Medical Chemistry, Westfälischen Wilhelms-Universität Münster, 48149, Münster, Germany.
Hyun-Jin ShinCollege of Veterinary Medicine, Chungnam National University, Daejeon, 34134, Republic of Korea.
Jae-Chul PyunDepartment of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea. jcpyun@yonsei.ac.kr.

Funding

Korea Health Industry Development Institute RS-2025-02213534National Research Foundation of Korea NRF-2020R1A5A101913111National Research Foundation of Korea RS-2023-00209053National Research Foundation of Korea RS-2024-00401422
6 · The paper itself

Abstract

Mimotopes of the porcine epidemic diarrhea virus (PEDV) spike protein (SP) were screened from a Fv-antibody library, and the neutralizing activity of screened mimotopes was analyzed using plaque assay and docking simulation. The screened Fv-antibody (mimotopes) corresponded to the variable region of the heavy chain of immunoglobulin G, composed of three complementarity-determining regions (CDRs) and four framework regions. The Fv-antibody library was constructed by randomizing the amino acid sequence of CDR3 and expressed on the outer membrane of E. coli using auto-display technology. Monoclonal anti-PEDV SP antibody was used as probes to screen Fv-antibodies mimicking PEDV SP from the library. Two screened Fv-antibodies (mimotopes of PEDV SP) with binding affinity to the monoclonal antibody were expressed as soluble proteins, and their binding affinity was estimated using a surface plasmon resonance biosensor. The neutralizing activity of PEDV SP mimotopes to prevent PEDV infection was calculated using a plaque assay based on the cytopathic effect. Additionally, molecular docking simulations were performed to examine the interactions of PEDV SP mimotopes with ACE2 receptor as well as APN which had been considered infection-related receptors for coronavirus.

Indexed as

Antibodies, NeutralizingAntibodies, ViralCoronavirus InfectionsPorcine epidemic diarrhea virusSpike Glycoprotein, CoronavirusAnimalsAntibodies, MonoclonalMolecular Docking SimulationNeutralization TestsSwineSwine DiseasesAntibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralSpike Glycoprotein, CoronavirusFv-antibody libraryMimotopeNeutralizing activityPorcine epidemic diarrhea virus (PEDV)

Identifiers

PMID41593368

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.