Differences between Scaly-sided Merganser (Mergus squamatus) and Common Merganser (M. merganser) feather microstructure.

The microstructural characteristics of feathers are useful for species identification. In this study, scanning electron microscopy was employed to examine the microstructures of contour feathers, rectrices, and down feathers from both the Scaly-sided Merganser (Mergus squamatus) and Common Merganser (Mergus merganser). The primary objective was to assess inter-species differences and evaluate the potential of these microstructural characteristics as reliable indicators for distinguishing species. Several microstructural characteristics of feathers exhibited significant variations between the two species. In rectrices, significant variations were observed in the prong length, base length, hooklet number, and prong number of distal barbules. Similarly, down feathers exhibited marked differences in the node number, distance between nodes, internode width, and barbule length of downy barbules. Stepwise discriminant analysis, combined with the leave-one-out cross-validation test, further validated the discriminatory power of all microstructural characteristics. For contour feathers, incorporating the base length into the model achieved a 56.9% correct classification rate. In rectrices, the hooklet number and prong length emerged as key discriminators, with a correct classification rate of 91.3%. Most notably, the barbule length, node number, and distance between nodes of down feathers demonstrated exceptional discriminative capabilities, attaining a perfect 100% correct classification rate. Consequently, the barbule length, node number, and distance between nodes of down feathers, may serve as potentially useful morphological markers for differentiating the Scaly-sided Merganser from the Common Merganser.