ArticleJournal of materials chemistry. B2026
Systematic investigation of the effects of neural stem cell spheroid size and density on fate specification in 3D culture.
Article in Journal of materials chemistry. B, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Bubble Formation Control: Fabrication of Centimeter-Sized Tissue-Like Constructs by Catalase-Coated Oxygen-Releasing Hydrogel.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Integrating single-cell and spheroid strategies in tissue engineering: Comparative insights from pancreas, cartilage, heart and brain.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
A systematic understanding of how the density of neural stem/progenitor cells (NS/PCs) embedded within three-dimensional (3D) biomaterials affect cell behavior will be necessary for developing effective strategies to generate CNS tissues. Here, we investigated the effects of local and global cell density of mouse neural stem cells (mNSCs) on their viability, proliferation, and differentiation when cultured in 3D, hyaluronic acid (HA)-based hydrogel matrices. Specifically, we assessed the influence of spheroid size, which represents local cell density, (small: 100 cells per sphere, large: 200 cells per sphere) and seeding density (low: 100 000 cells per hydrogel, high: 200 000 cells per hydrogel), which represents global density, on cellular outcomes. Results reveal that these factors have both independent and interactive effects on NS/PC viability and fate. Cultures of smaller spheres at low global densities yield more glial cells, including astrocytes and oligodendrocytes. In contrast, cultures with high global densities, regardless of sphere size, better preserved stem-like mNSC phenotypes. Strikingly, cultures with 1000 total spheres per hydrogel, regardless of sphere size or overall cell concentration, best maintained viability while promoting neuronal maturation. These findings highlight the importance of controlling both local and global cell densities in 3D cultures to achieve reproducible mNSC-derived populations for use as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.