Systematic Approach to Reducing Errors in Deoxynivalenol Quantification: Insights from Bulk Wheat Sampling and Sample Preparation.

Accurate quantification of Deoxynovalienol (DON) in wheat is critical for food safety, but current methods suffer from poor reproducibility due to inconsistent operational parameters across the sampling and analysis workflow. To address this issue, this study focused on truck-loaded bulk wheat and conducted a comprehensive analysis covering the entire process from sampling to laboratory testing. By examining parameters at each stage-test portion, laboratory sample, composite sample, and primary sample-and applying the Monte Carlo simple random sampling principle, the variability associated with the full-process parameters for DON detection in wheat was systematically analyzed. The errors introduced at each step were evaluated, leading to the development of a representative measurement procedure for DON in truck-loaded bulk wheat. The results indicate that for truck-loaded bulk wheat, sampling should be conducted using a random distribution method with no fewer than 11 sampling points, each providing a primary sample of at least 500 g. The composite sample should be homogenized three times using a cone-and-quartering divider before subsampling. The laboratory sample should weigh no less than 750 g and be ground to a particle size of 1 mm. After thorough mixing of the ground sample, 5 g should be accurately weighed for analysis. This measurement procedure introduces a total relative error of 12.9%. The proposed protocol for DON detection in truck-loaded wheat offers a practical approach that minimizes error contribution from each parameter while maintaining low economic and time costs, ensuring feasibility for field implementation.